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UV-C Light-Based Surface Disinfection: Analysis of Its Virucidal Efficacy Using a Bacteriophage Model.
Rudhart, Stefan A; Günther, Frank; Dapper, Laura; Stuck, Boris A; Hoch, Stephan.
  • Rudhart SA; Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany.
  • Günther F; Department of Medical Microbiology and Hygiene, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany.
  • Dapper L; Department of Medical Microbiology and Hygiene, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany.
  • Stuck BA; Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany.
  • Hoch S; Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany.
Int J Environ Res Public Health ; 19(6)2022 03 10.
Article in English | MEDLINE | ID: covidwho-1742427
ABSTRACT

BACKGROUND:

The reprocessing of medical devices has become more complex due to increasing hygiene requirements. Previous studies showed satisfactory bactericidal disinfection effects of UV-C light in rigid and flexible endoscopes. Especially in the context of the current COVID-19 pandemic, virucidal properties are of high importance. In the present study, the virucidal efficacy of UV-C light surface disinfection was analyzed.

METHODS:

MS-2 bacteriophages were applied to the test samples and irradiated by UV-C light using the UV Smart D25 device; unirradiated test samples were used as controls. A dilution series of the samples was mixed with 1 × 108 Escherichia coli and assayed.

RESULTS:

8.6 × 1012 pfu could be harvested from the unprocessed test samples. In the control group without UV-C exposure, a remaining contamination of 1.2 × 1012 pfu was detected, resulting in a procedural baseline reduction rate with a LOG10 reduction factor of 0.72. The LOG10 reduction factor was found to be 3.0 after 25 s of UV-C light exposure. After 50 and 75 s of UV-C radiation LOG10 reduction factors 4.2 and 5.9, respectively, were found, with all reductions being statistically significantly different to baseline.

CONCLUSIONS:

The tested UV system seems to provide a significant virucidal effect after a relatively short irradiation time.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bacteriophages / COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijerph19063246

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bacteriophages / COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijerph19063246