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No substantial preexisting B cell immunity against SARS-CoV-2 in healthy adults.
Ercanoglu, Meryem Seda; Gieselmann, Lutz; Dähling, Sabrina; Poopalasingam, Nareshkumar; Detmer, Susanne; Koch, Manuel; Korenkov, Michael; Halwe, Sandro; Klüver, Michael; Di Cristanziano, Veronica; Janicki, Hanna; Schlotz, Maike; Worczinski, Johanna; Gathof, Birgit; Gruell, Henning; Zehner, Matthias; Becker, Stephan; Vanshylla, Kanika; Kreer, Christoph; Klein, Florian.
  • Ercanoglu MS; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Gieselmann L; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Dähling S; German Center for Infection Research, Partner Site Bonn-Cologne, 50931 Cologne, Germany.
  • Poopalasingam N; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Detmer S; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Koch M; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Korenkov M; Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany.
  • Halwe S; Institute for Dental Research and Oral Musculoskeletal Biology and Center for Biochemistry, University of Cologne, 50931 Cologne, Germany.
  • Klüver M; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Di Cristanziano V; Institute of Virology, Philipps University Marburg, Hans-Meerwein-Straße 2, 35042 Marburg, Germany.
  • Janicki H; German Center for Infection Research, Partner Site Giessen-Marburg-Langen, 35043 Marburg, Germany.
  • Schlotz M; Institute of Virology, Philipps University Marburg, Hans-Meerwein-Straße 2, 35042 Marburg, Germany.
  • Worczinski J; German Center for Infection Research, Partner Site Giessen-Marburg-Langen, 35043 Marburg, Germany.
  • Gathof B; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Gruell H; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Zehner M; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Becker S; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Vanshylla K; Institute of Transfusion Medicine, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, Germany.
  • Kreer C; Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
  • Klein F; German Center for Infection Research, Partner Site Bonn-Cologne, 50931 Cologne, Germany.
iScience ; 25(3): 103951, 2022 Mar 18.
Article in English | MEDLINE | ID: covidwho-1747872
ABSTRACT
Preexisting immunity against SARS-CoV-2 may have critical implications for our understanding of COVID-19 susceptibility and severity. The presence and clinical relevance of a preexisting B cell immunity remain to be fully elucidated. Here, we provide a detailed analysis of the B cell immunity to SARS-CoV-2 in unexposed individuals. To this end, we extensively investigated SARS-CoV-2 humoral immunity in 150 adults sampled pre-pandemically. Comprehensive screening of donor plasma and purified IgG samples for binding and neutralization in various functional assays revealed no substantial activity against SARS-CoV-2 but broad reactivity to endemic betacoronaviruses. Moreover, we analyzed antibody sequences of 8,174 putatively SARS-CoV-2-reactive B cells at a single cell level and generated and tested 158 monoclonal antibodies. None of these antibodies displayed relevant binding or neutralizing activity against SARS-CoV-2. Taken together, our results show no evidence of competent preexisting antibody and B cell immunity against SARS-CoV-2 in unexposed adults.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Journal: IScience Year: 2022 Document Type: Article Affiliation country: J.isci.2022.103951

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Journal: IScience Year: 2022 Document Type: Article Affiliation country: J.isci.2022.103951