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Comparison of reverse-transcription qPCR and droplet digital PCR for the detection of SARS-CoV-2 in clinical specimens of hospitalized patients.
Li, Jingyuan; Lin, Weishi; Du, Pibo; Liu, Wei; Liu, Xiong; Yang, Chaojie; Jia, Ruizhong; Wang, Yong; Chen, Yong; Jia, Leili; Han, Li; Tan, Weilong; Liu, Nan; Du, Junjie; Ke, Yuehua; Wang, Changjun.
  • Li J; Department of Orthopaedics, Air Force Clinical College (Air Force Medical Center) of Anhui Medical University, Beijing, P. R. China.
  • Lin W; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Du P; Chinese PLA 910 Hospital, Quanzhou, Fujian Province, China.
  • Liu W; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Liu X; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Yang C; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Jia R; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Wang Y; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Chen Y; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Jia L; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Han L; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China.
  • Tan W; Huadong Research Institute for Medicine and Biotechniques, Nanjing, Jiangsu Province, China.
  • Liu N; Chinese PLA 910 Hospital, Quanzhou, Fujian Province, China.
  • Du J; Department of Orthopaedics, Air Force Clinical College (Air Force Medical Center) of Anhui Medical University, Beijing, P. R. China. Electronic address: dujunjie205@hotmail.com.
  • Ke Y; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China. Electronic address: yuehuakebj@163.com.
  • Wang C; Chinese PLA Center for Disease Control and Prevention, Beijing, China; Wuhan Huoshenshan Hospital, Wuhan, Hubei Province, China. Electronic address: science2008@hotmail.com.
Diagn Microbiol Infect Dis ; 103(2): 115677, 2022 Jun.
Article in English | MEDLINE | ID: covidwho-1748081
ABSTRACT
Accurate detection of severe acute respiratory syndrome coronavirus 2 is not only necessary for viral load monitoring to optimize treatment in hospitalized coronavirus disease 2019 patients, but also critical for deciding whether the patient could be discharged without any risk of viral shedding. Digital droplet PCR (ddPCR) is more sensitive than reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and is usually considered the superior choice. In the current study, we compared the clinical performance of RT-qPCR and ddPCR using oropharyngeal swab samples from patients hospitalized in the temporary Huoshenshan Hospital, Wuhan, Hubei, China. Results demonstrated that ddPCR was indeed more sensitive than RT-qPCR. Negative results might be caused by poor sampling technique or recovered patients, as the range of viral load in these patients varied significantly. In addition, both methods were highly correlated in terms of their ability to detect all three target genes as well as the ratio of copies of viral genes to that of the IC gene. Furthermore, our results evidenced that both methods detected the N gene more easily than the ORF gene. Taken together, these findings imply that the use of ddPCR, as an alternative to RT-qPCR, is necessary for the accurate diagnosis of hospitalized coronavirus disease 2019 patients.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article