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Argonaute-integrated isothermal amplification for rapid, portable, multiplex detection of SARS-CoV-2 and influenza viruses.
Ye, Xingyu; Zhou, Haiwei; Guo, Xiang; Liu, Donglai; Li, Zhonglei; Sun, Junwei; Huang, Jun; Liu, Tao; Zhao, Pengshu; Xu, Heshan; Li, Kai; Wang, Hanming; Wang, Jihua; Wang, Li; Zhao, Weili; Liu, Qian; Xu, Sihong; Feng, Yan.
  • Ye X; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Zhou H; National Institutes for Food and Drug Control, Beijing, 100050, China.
  • Guo X; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Liu D; National Institutes for Food and Drug Control, Beijing, 100050, China.
  • Li Z; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Sun J; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Huang J; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China.
  • Liu T; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Zhao P; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Xu H; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Li K; GeneTalks Biotechnology Inc., Changsha, 410013, China.
  • Wang H; Guangdong Medical Laboratory Technology (Rapid Diagnostic) Engineering Technology Research Center, Guangzhou, 510641, China; Guangzhou Wondfo Biotech Co., Ltd., Guangzhou, 510641, China.
  • Wang J; Guangdong Medical Laboratory Technology (Rapid Diagnostic) Engineering Technology Research Center, Guangzhou, 510641, China; Guangzhou Wondfo Biotech Co., Ltd., Guangzhou, 510641, China.
  • Wang L; Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University of Medicine, Shanghai, 200025, China.
  • Zhao W; Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University of Medicine, Shanghai, 200025, China.
  • Liu Q; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: liuqian1018@sjtu.edu.cn.
  • Xu S; National Institutes for Food and Drug Control, Beijing, 100050, China. Electronic address: xushong@nifdc.org.cn.
  • Feng Y; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: yfeng2009@sjtu.edu.cn.
Biosens Bioelectron ; 207: 114169, 2022 Jul 01.
Article in English | MEDLINE | ID: covidwho-1748198
ABSTRACT
Isothermal amplification methods are a promising trend in virus detection because of their superiority in rapidity and sensitivity. However, the generation of false positives and limited multiplexity are major bottlenecks that must be addressed. In this study, we developed a multiplex Argonaute (Ago)-based nucleic acid detection system (MULAN) that integrates rapid isothermal amplification with the multiplex inclusiveness of a single Ago for simultaneous detection of multiple targets such as SARS-CoV-2 and influenza viruses. Owing to its high specificity, MULAN can distinguish targets at a single-base resolution for mutant genotyping. Moreover, MULAN also supports portable and visible devices with a limit of detection of five copies per reaction. Validated by SARS-CoV-2 pseudoviruses and clinical samples of influenza viruses, MULAN showed 100% agreement with quantitative reverse-transcription PCR. These results demonstrated that MULAN has great potential to facilitate reliable, easy, and quick point-of-care diagnosis for promoting the control of infectious diseases.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Orthomyxoviridae / Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2022.114169

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Orthomyxoviridae / Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2022.114169