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ChAdOx1-S adenoviral vector vaccine applied intranasally elicits superior mucosal immunity compared to the intramuscular route of vaccination.
Cokaric Brdovcak, Maja; Materljan, Jelena; Sustic, Marko; Ravlic, Sanda; Ruzic, Tina; Lisnic, Berislav; Miklic, Karmela; Brizic, Ilija; Pribanic Matesic, Marina; Juranic Lisnic, Vanda; Halassy, Beata; Roncevic, Dobrica; Knezevic, Zdravka; Stefan, Leo; Bertoglio, Federico; Schubert, Maren; Cicin-Sain, Luka; Markotic, Alemka; Jonjic, Stipan; Krmpotic, Astrid.
  • Cokaric Brdovcak M; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Materljan J; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Sustic M; Department of Histology and Embryology, University of Rijeka, Rijeka, Croatia.
  • Ravlic S; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Ruzic T; Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Zagreb, Croatia.
  • Lisnic B; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Miklic K; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Brizic I; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Pribanic Matesic M; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Juranic Lisnic V; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Halassy B; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • Roncevic D; Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Zagreb, Croatia.
  • Knezevic Z; Teaching Institute of Public Health PGC, Rijeka, Croatia.
  • Stefan L; JGL d.d. Jadran Galenski Laboratorij, Rijeka, Croatia.
  • Bertoglio F; JGL d.d. Jadran Galenski Laboratorij, Rijeka, Croatia.
  • Schubert M; Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Braunschweig, Germany.
  • Cicin-Sain L; Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Braunschweig, Germany.
  • Markotic A; Department of Viral Immunology, Helmholtz Center for Infection Research, Braunschweig, Germany.
  • Jonjic S; Research Department, University Hospital for Infectious Diseases "Fran Mihaljevic", Zagreb, Croatia.
  • Krmpotic A; Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
Eur J Immunol ; 52(6): 936-945, 2022 06.
Article in English | MEDLINE | ID: covidwho-1750364
ABSTRACT
COVID-19 vaccines prevent severe forms of the disease, but do not warrant complete protection against breakthrough infections. This could be due to suboptimal mucosal immunity at the site of virus entry, given that all currently approved vaccines are administered via the intramuscular route. In this study, we assessed humoral and cellular immune responses in BALB/c mice after intranasal and intramuscular immunization with adenoviral vector ChAdOx1-S expressing full-length Spike protein of SARS-CoV-2. We showed that both routes of vaccination induced a potent IgG antibody response, as well as robust neutralizing capacity, but intranasal vaccination elicited a superior IgA antibody titer in the sera and in the respiratory mucosa. Bronchoalveolar lavage from intranasally immunized mice efficiently neutralized SARS-CoV-2, which has not been the case in intramuscularly immunized group. Moreover, substantially higher percentages of epitope-specific CD8 T cells exhibiting a tissue resident phenotype were found in the lungs of intranasally immunized animals. Finally, both intranasal and intramuscular vaccination with ChAdOx1-S efficiently protected the mice after the challenge with recombinant herpesvirus expressing the Spike protein. Our results demonstrate that intranasal application of adenoviral vector ChAdOx1-S induces superior mucosal immunity and therefore could be a promising strategy for putting the COVID-19 pandemic under control.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Type of study: Observational study Topics: Vaccines Limits: Animals / Humans Language: English Journal: Eur J Immunol Year: 2022 Document Type: Article Affiliation country: Eji.202249823

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Type of study: Observational study Topics: Vaccines Limits: Animals / Humans Language: English Journal: Eur J Immunol Year: 2022 Document Type: Article Affiliation country: Eji.202249823