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Inhaled Placental Mesenchymal Stromal Cell Secretome from Two- and Three-Dimensional Cell Cultures Promotes Survival and Regeneration in Acute Lung Injury Model in Mice.
Kudinov, Vasily A; Artyushev, Rafael I; Zurina, Irina M; Zorina, Elena S; Lapshin, Roman D; Snopova, Ludmila B; Mukhina, Irina V; Saburina, Irina N.
  • Kudinov VA; Laboratory of Cell Biology and Developmental Pathology, FSBSI Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Artyushev RI; Laboratory of Cell Biology and Developmental Pathology, FSBSI Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Zurina IM; Laboratory of Cell Biology and Developmental Pathology, FSBSI Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Zorina ES; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St., 119991 Moscow, Russia.
  • Lapshin RD; Postgenomic Data Analysis Laboratory, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
  • Snopova LB; Central Research Laboratory, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, Russia.
  • Mukhina IV; Central Research Laboratory, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, Russia.
  • Saburina IN; Central Research Laboratory, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, Russia.
Int J Mol Sci ; 23(7)2022 Mar 22.
Article in English | MEDLINE | ID: covidwho-1753510
ABSTRACT
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) is a common clinical problem, leading to significant morbidity and mortality, and no effective pharmacotherapy exists. The problem of ARDS causing mortality became more apparent during the COVID-19 pandemic. Biotherapeutic products containing multipotent mesenchymal stromal cell (MMSC) secretome may provide a new therapeutic paradigm for human healthcare due to their immunomodulating and regenerative abilities. The content and regenerative capacity of the secretome depends on cell origin and type of cultivation (two- or three-dimensional (2D/3D)). In this study, we investigated the proteomic profile of the secretome from 2D- and 3D-cultured placental MMSC and lung fibroblasts (LFBs) and the effect of inhalation of freeze-dried secretome on survival, lung inflammation, lung tissue regeneration, fibrin deposition in a lethal ALI model in mice. We found that three inhaled administrations of freeze-dried secretome from 2D- and 3D-cultured placental MMSC and LFB protected mice from death, restored the histological structure of damaged lungs, and decreased fibrin deposition. At the same time, 3D MMSC secretome exhibited a more pronounced trend in lung recovery than 2D MMSC and LFB-derived secretome in some measures. Taking together, these studies show that inhalation of cell secretome may also be considered as a potential therapy for the management of ARDS in patients suffering from severe pneumonia, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), however, their effectiveness requires further investigation.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia / Respiratory Distress Syndrome / Mesenchymal Stem Cell Transplantation / Acute Lung Injury / Mesenchymal Stem Cells / COVID-19 Type of study: Prognostic study Limits: Animals / Female / Humans / Pregnancy Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23073417

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia / Respiratory Distress Syndrome / Mesenchymal Stem Cell Transplantation / Acute Lung Injury / Mesenchymal Stem Cells / COVID-19 Type of study: Prognostic study Limits: Animals / Female / Humans / Pregnancy Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23073417