An economical and sensitive glassmilk-based nucleic-acid extraction approach for SARS-CoV-2 diagnostics
STAR protocols
; 2022.
Article
in English
| EuropePMC | ID: covidwho-1755971
ABSTRACT
The gold standard protocol for SARS-CoV-2 infection detection remains reverse transcription quantitative polymerase chain reaction (RT-qPCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high throughput settings. Graphical
Search on Google
Collection:
Databases of international organizations
Database:
EuropePMC
Language:
English
Journal:
STAR protocols
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS