Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.
Cells
; 11(6)2022 03 08.
Article
in English
| MEDLINE | ID: covidwho-1760407
ABSTRACT
A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Lysosomal Storage Diseases
/
Ion Channels
Type of study:
Experimental Studies
Limits:
Humans
Language:
English
Year:
2022
Document Type:
Article
Affiliation country:
Cells11060921
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