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Rational Development of a Polysaccharide-Protein-Conjugated Nanoparticle Vaccine Against SARS-CoV-2 Variants and Streptococcus pneumoniae.
Deng, Yongqiang; Li, Jing; Sun, Chunyun; Chi, Hang; Luo, Dan; Wang, Rui; Qiu, Hongying; Zhang, Yanjing; Wu, Mei; Zhang, Xiao; Huang, Xun; Xie, Liangzhi; Qin, Chengfeng.
  • Deng Y; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, P. R. China.
  • Li J; Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Beijing, 100176, P. R. China.
  • Sun C; Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Beijing, 100176, P. R. China.
  • Chi H; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, P. R. China.
  • Luo D; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, P. R. China.
  • Wang R; Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Beijing, 100176, P. R. China.
  • Qiu H; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, P. R. China.
  • Zhang Y; Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Beijing, 100176, P. R. China.
  • Wu M; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, P. R. China.
  • Zhang X; Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Beijing, 100176, P. R. China.
  • Huang X; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, P. R. China.
  • Xie L; Beijing Protein and Antibody R&D Engineering Center, Sinocelltech Ltd., Beijing, 100176, P. R. China.
  • Qin C; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, P. R. China.
Adv Mater ; 34(21): e2200443, 2022 May.
Article in English | MEDLINE | ID: covidwho-1763176
ABSTRACT
The ongoing COVID-19 pandemic caused by SARS-CoV-2 has led to millions of deaths worldwide. Streptococcus pneumoniae (S. pneumoniae) remains a major cause of mortality in underdeveloped countries. A vaccine that prevents both SARS-CoV-2 and S. pneumoniae infection represents a long-sought "magic bullet". Herein, a nanoparticle vaccine, termed SCTV01B, is rationally developed by using the capsular polysaccharide of S. pneumoniae serotype 14 (PPS14) as the backbone to conjugate with the recombinant receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. The final formulation of conjugated nanoparticles in the network structure exhibits high thermal stability. Immunization with SCTV01B induces potent humoral and Type 1/Type 2 T helper cell (Th1/Th2) cellular immune responses in mice, rats, and rhesus macaques. In particular, SCTV01B-immunized serum not only broadly cross-neutralizes all SARS-CoV-2 variants of concern (VOCs), including the most recent Omicron variant, but also shows high opsonophagocytic activity (OPA) against S. pneumoniae serotype 14. Finally, SCTV01B vaccination confers protection against challenges with the SARS-CoV-2 mouse-adapted strain and the original strain in established murine models. Collectively, these promising preclinical results support further clinical evaluation of SCTV01B, highlighting the potency of polysaccharide-RBD-conjugated nanoparticle vaccine platforms for the development of vaccines for COVID-19 and other infectious diseases.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines / Nanoparticles / COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Adv Mater Journal subject: Biophysics / Chemistry Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines / Nanoparticles / COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Adv Mater Journal subject: Biophysics / Chemistry Year: 2022 Document Type: Article