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HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.
Nudell, Victoria; Wang, Yu; Pang, Zhengyuan; Lal, Neeraj K; Huang, Min; Shaabani, Namir; Kanim, Wesam; Teijaro, John; Maximov, Anton; Ye, Li.
  • Nudell V; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA.
  • Wang Y; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA.
  • Pang Z; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA.
  • Lal NK; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA.
  • Huang M; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA.
  • Shaabani N; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA.
  • Kanim W; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA.
  • Teijaro J; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA.
  • Maximov A; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA.
  • Ye L; Department of Neuroscience, The Scripps Research Institute, La Jolla, CA, USA. liye@scripps.edu.
Nat Methods ; 19(4): 479-485, 2022 04.
Article in English | MEDLINE | ID: covidwho-1764194
ABSTRACT
The recent development of solvent- and polymer-based brain-clearing techniques has advanced our ability to visualize the mammalian nervous system in three dimensions. However, it remains challenging to image the mammalian body en bloc. Here we developed HYBRiD (hydrogel-based reinforcement of three-dimensional imaging solvent-cleared organs (DISCO)), by recombining components of organic- and polymer-based clearing pipelines. We achieved high transparency and protein retention, as well as compatibility with direct fluorescent imaging and immunostaining in cleared mammalian bodies. Using parvalbumin- and somatostatin-Cre models, we demonstrated the utility of HYBRiD for whole-body imaging of genetically encoded fluorescent reporters without antibody enhancement of signals in newborn and juvenile mice. Using K18-hACE2 transgenic mice, HYBRiD enabled perfusion-free clearing and visualization of SARS-CoV-2 infection in a whole mouse chest, revealing macroscopic and microscopic features of viral pathology in the same sample. HYBRiD offers a simple and universal solution to visualize large heterogeneous body parts or entire animals for basic and translational research.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Hydrogels / COVID-19 Limits: Animals Language: English Journal: Nat Methods Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: S41592-022-01427-0

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Hydrogels / COVID-19 Limits: Animals Language: English Journal: Nat Methods Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: S41592-022-01427-0