Spectrophotometric quantitative analysis of remdesivir using acid dye reagent selected by computational calculations.
Spectrochim Acta A Mol Biomol Spectrosc
; 276: 121188, 2022 Aug 05.
Article
in English
| MEDLINE | ID: covidwho-1768515
ABSTRACT
Remdesivir was approved by the Food and Drug Administration for the treatment of COVID -19 in hospitalized adult and pediatric patients. Application of computational calculations for choosing the sensitive reagent in spectrophotometric quantitative analysis is very limited. Computational and theoretical studies were used for choosing the best acid dye for selective visible spectrophotometric quantitative analysis of remdesivir. The calculations were performed using Gaussian 03 software with the density functional theory method using B3LYP/6-31G(d) basis set. The theoretical studies revealed that bromophenol blue is a better match for remdesivir than other acid dyes due to the higher calculated interaction energy. The proposed method was based on the reaction of remdesivir with the computationally selected acid dye bromophenol blue to form a yellow ion-pair complex. The spectra showed absorption peaks at 418 nm. Various factors affecting the reaction were optimized. The method was successfully applied for the determination of remdesivir in the pharmaceutical preparation with good accuracy and precision. Beer's law was observed in the concentration range of 2-12 µg/mL of remdesivir. The proposed reaction was used as a basis for the spectrophotometric determination of remdesivir in pure form and in the pharmaceutical preparation.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Coloring Agents
/
COVID-19 Drug Treatment
Limits:
Child
/
Humans
Country/Region as subject:
North America
Language:
English
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Journal subject:
Molecular Biology
Year:
2022
Document Type:
Article
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