The great value of clinical laboratories in the pandemic process
Turkish Journal of Biochemistry
; 46(SUPPL 2):85, 2021.
Article
in English
| EMBASE | ID: covidwho-1770806
ABSTRACT
Laboratory Medicine has been showing its great Value during Covid-19 pandemic in the whole care process diagnosis, screening, follow up and outcome of the disease. The diagnostic tools to date are able to dose viral RNA, and antibodies against viral proteins or viral proteins themselves. Even if the conventional RT-PCR has been the most widely used method, the greatest innovation in molecular diagnosis was reported by the so called CRISPR Community. The first serological tests to be introduced were rapid serological tests with direct reading (first generation) or with fluorescence reading (2nd generation) and microfluidic with fluorescent reading (3rd generation). Then conventional CLIA method have been introduced with better sensitivity and more recently a new kind of serological assay has been proposed able to detect anti SARS-Cov-2 serum antibodies throughout a competitive streptoavidin/biotin assay which utilize RBD as coated antigen and ACE-2 labelled with biotin as competitor molecule for serum antibodies. Until few months ago the harmonization between many methods produced by many manufacturers was impossible. Recently the WHO produced an international standard (pool of sera-total antibodies) able to permit a sort of harmonization. To date NGS return to be widely utilized in the effort to intercept new variants. Finally, pathophysiology and natural course of Post-acute Sequelae of COVID-19 (PASC) also called Long Covidis unclear, meriting further studies. Also in this almost still unknown field, Clinical Laboratory could contribute in diagnosis and monitoring.
antigen; biotin; dipeptidyl carboxypeptidase; endogenous compound; viral protein; virus RNA; antibody blood level; chemiluminescence immunoassay; clinical laboratory; clustered regularly interspaced short palindromic repeat; complication; conference abstract; controlled study; coronavirus disease 2019; diagnosis; fluorescence; follow up; human; human tissue; intermethod comparison; molecular diagnosis; nonhuman; pandemic; serology; Severe acute respiratory syndrome coronavirus 2; standard
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Type of study:
Prognostic study
Language:
English
Journal:
Turkish Journal of Biochemistry
Year:
2021
Document Type:
Article
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