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Detection and Kinetics of Subgenomic Severe Acute Respiratory Syndrome Coronavirus 2 RNA Viral Load in Longitudinal Diagnostic RNA-Positive Samples.
Deming, Meagan E; Dong, Tracy Q; Agrawal, Vaidehi; Mills, Margaret G; Huang, Meei Li W; Greninger, Alexander L; Jerome, Keith R; Wener, Mark H; Paasche-Orlow, Michael K; Kissinger, Patricia; Luk, Alfred; Hoffman, Risa M; Stewart, Jenell; Kottkamp, Angelica C; Bershteyn, Anna; Chu, Helen Y; Stankiewicz Karita, Helen C; Johnston, Christine M; Wald, Anna; Barnabas, Ruanne; Brown, Elizabeth R; Neuzil, Kathleen M.
  • Deming ME; The Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Dong TQ; Vaccine and Infectious Disease Division, Fred Hutchinson, Cancer Research Center, Seattle, Washington, USA.
  • Agrawal V; The Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Mills MG; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
  • Huang MLW; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
  • Greninger AL; Vaccine and Infectious Disease Division, Fred Hutchinson, Cancer Research Center, Seattle, Washington, USA.
  • Jerome KR; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
  • Wener MH; Vaccine and Infectious Disease Division, Fred Hutchinson, Cancer Research Center, Seattle, Washington, USA.
  • Paasche-Orlow MK; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
  • Kissinger P; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
  • Luk A; Division of Rheumatology, Department of Medicine, University of Washington, Seattle, Washington, USA.
  • Hoffman RM; Section of General Internal Medicine, Department of Medicine, Boston University School of Medicine and Boston Medical Center, Boston, Massachusetts, USA.
  • Stewart J; Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine.
  • Kottkamp AC; Section of Infectious Diseases, John W. Deming, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana, USA.
  • Bershteyn A; Division of Infectious Diseases, David Geffen School of Medicine at the University of California, Los Angeles, California, USA.
  • Chu HY; Department of Medicine, University of Washington, Seattle, Washington, USA.
  • Stankiewicz Karita HC; Division of Infectious Diseases & Immunology, Department of Medicine, New York University Grossman, School of Medicine, New York, New York, USA.
  • Johnston CM; Department of Population Health; New York University, Grossman School of Medicine, New York, New York, USA.
  • Wald A; Department of Epidemiology, University of Washington, Seattle, Washington, USA.
  • Barnabas R; Division of Infectious Diseases & Immunology, Department of Medicine, New York University Grossman, School of Medicine, New York, New York, USA.
  • Brown ER; Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
  • Neuzil KM; Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
J Infect Dis ; 226(5): 788-796, 2022 09 13.
Article in English | MEDLINE | ID: covidwho-1774394
ABSTRACT
While detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by diagnostic reverse-transcription polymerase chain reaction (RT-PCR) is highly sensitive for viral RNA, the nucleic acid amplification of subgenomic RNAs (sgRNAs) that are the product of viral replication may more accurately identify replication. We characterized the diagnostic RNA and sgRNA detection by RT-PCR from nasal swab samples collected daily by participants in postexposure prophylaxis or treatment studies for SARS-CoV-2. Among 1932 RT-PCR-positive swab samples with sgRNA tests, 40% (767) had detectable sgRNA. Above a diagnostic RNA viral load (VL) threshold of 5.1 log10 copies/mL, 96% of samples had detectable sgRNA with VLs that followed a linear trend. The trajectories of diagnostic RNA and sgRNA VLs differed, with 80% peaking on the same day but duration of sgRNA detection being shorter (8 vs 14 days). With a large sample of daily swab samples we provide comparative sgRNA kinetics and a diagnostic RNA threshold that correlates with replicating virus independent of symptoms or duration of illness.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: J Infect Dis Year: 2022 Document Type: Article Affiliation country: Infdis

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: J Infect Dis Year: 2022 Document Type: Article Affiliation country: Infdis