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The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors.
Kroemer, Marie; Boullerot, Laura; Ramseyer, Mélanie; Spehner, Laurie; Barisien, Christophe; Gravelin, Eleonore; Renaudin, Adeline; Cognasse, Fabrice; Gallian, Pierre; Hermine, Olivier; Lacombe, Karine; Tiberghien, Pierre; Adotévi, Olivier.
  • Kroemer M; INSERM, EFS BFC, UMR1098, RIGHT, University of Burgundy Franche-Comte, Besançon, France.
  • Boullerot L; Department of Pharmacy, University Hospital of Besançon, Besançon, France.
  • Ramseyer M; INSERM, EFS BFC, UMR1098, RIGHT, University of Burgundy Franche-Comte, Besançon, France.
  • Spehner L; INSERM CIC1431, Clinical Investigation Center in Biotherapy, Biomonitoring Plateform, Besançon, France.
  • Barisien C; INSERM CIC1431, Clinical Investigation Center in Biotherapy, Biomonitoring Plateform, Besançon, France.
  • Gravelin E; INSERM, EFS BFC, UMR1098, RIGHT, University of Burgundy Franche-Comte, Besançon, France.
  • Renaudin A; Department of Medical Oncology, Biotechnology and Immune-Oncology Platforme, University Hospital of Besançon, Besançon, France.
  • Cognasse F; Établissement Français du Sang Bourgogne Franche-Comté, Besançon, France.
  • Gallian P; INSERM CIC1431, Clinical Investigation Center in Biotherapy, Biomonitoring Plateform, Besançon, France.
  • Hermine O; INSERM CIC1431, Clinical Investigation Center in Biotherapy, Biomonitoring Plateform, Besançon, France.
  • Lacombe K; Établissement Français du Sang Auvergne-Rhône-Alpes, Saint-Etienne, France.
  • Tiberghien P; SAINBIOSE, INSERM, U1059, University of Lyon, Saint-Etienne, France.
  • Adotévi O; Établissement Français du Sang, La Plaine St-Denis, France.
Front Public Health ; 10: 816848, 2022.
Article in English | MEDLINE | ID: covidwho-1776012
ABSTRACT
Convalescent plasma therapy has been described as an attractive approach to treat critically ill patients with COVID-19 (Coronavirus disease 2019). The selection of convalescent plasma donors (CPD) is commonly based on neutralizing antibody titer. A better understanding of the quality of immune responses following COVID-19 will enable the optimization of convalescent donors' selection in convalescent plasma programs. The involvement of SARS-CoV-2 specific T cells in the induction and persistence of high affinity anti-SARS-CoV-2 neutralizing antibody is still poorly investigated. In this study, 115 CPD who presented SARS-CoV-2 and who were eligible for plasma donation were included. Comprehensive analysis of T cells together with humoral responses were performed in regards of sex, age and blood group type. High frequency of T cell responses against SARS-CoV-2 related protein such as spike glycoprotein (80.0%), nucleocapsid (NCAP) (70.4%) and membrane protein (VME1) (74.8%) were detected in CPD by ex vivo IFN-γ and TNF-α ELISpot assays. Among CPD responders, most exhibited poly-specific T cell responses (75%) defined by the ability to mount responses against at least two SARS-CoV-2 antigens. We found a positive correlation between the magnitude and the poly-specificity of anti-SARS-CoV-2 T cell responses in CPD. Notably, both the magnitude and poly-specificity of SARS-CoV-2 T cell responses were highly correlated with neutralizing antibody titer in CPD. The present study highlights that the poly-specificity and strength of SARS-CoV-2 specific T cell responses predicts neutralizing antibody titer following COVID-19. These observations show the interest to combine T cell assays and antibody titer for the selection of CPD and to a latter extend to assess COVID-19 vaccine efficacy in at-risk patients.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Front Public Health Year: 2022 Document Type: Article Affiliation country: Fpubh.2022.816848

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Front Public Health Year: 2022 Document Type: Article Affiliation country: Fpubh.2022.816848