Your browser doesn't support javascript.
Cytokine Profile and Anti-Inflammatory Activity of a Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells (PRS CK STORM).
Lapuente, Juan Pedro; Blázquez-Martínez, Alejandro; Marco-Brualla, Joaquín; Gómez, Gonzalo; Desportes, Paula; Sanz, Jara; Fernández, Pablo; García-Gil, Mario; Bermejo, Fernando; San Martín, Juan V; Algaba, Alicia; De Gregorio, Juan Carlos; Lapuente, Daniel; De Gregorio, Almudena; Lapuente, Belén; Andrés, María de la Viñas; Anel, Alberto.
  • Lapuente JP; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Blázquez-Martínez A; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Marco-Brualla J; Group Immunity, Cancer and Stem Cells, Faculty of Sciences, University of Zaragoza, 50009 Zaragoza, Spain.
  • Gómez G; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Desportes P; GMP Facility, Peaches Biotech, 28050 Madrid, Spain.
  • Sanz J; GMP Facility, Peaches Biotech, 28050 Madrid, Spain.
  • Fernández P; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • García-Gil M; Pharmacy Department, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Bermejo F; Digestive Department, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • San Martín JV; Medicine Department, University Rey Juan Carlos, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Algaba A; Internal Medicine Department, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • De Gregorio JC; Clinical Assay Department, Fuelabrada Hospital, 28942 Madrid, Spain.
  • Lapuente D; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • De Gregorio A; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Lapuente B; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Andrés MV; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
  • Anel A; R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain.
Biomolecules ; 12(4)2022 03 31.
Article in English | MEDLINE | ID: covidwho-1776123
ABSTRACT
Intercellular communication between monocytes/macrophages and cells involved in tissue regeneration, such as mesenchymal stromal cells (MSCs) and primary tissue cells, is essential for tissue regeneration and recovery of homeostasis. Typically, in the final phase of the inflammation-resolving process, this intercellular communication drives an anti-inflammatory immunomodulatory response. To obtain a safe and effective treatment to counteract the cytokine storm associated with a disproportionate immune response to severe infections, including that associated with COVID-19, by means of naturally balanced immunomodulation, our group has standardized the production under GMP-like conditions of a secretome by coculture of macrophages and MSCs. To characterize this proteome, we determined the expression of molecules related to cellular immune response and tissue regeneration, as well as its possible toxicity and anti-inflammatory potency. The results show a specific molecular pattern of interaction between the two cell types studied, with an anti-inflammatory and regenerative profile. In addition, the secretome is not toxic by itself on human PBMC or on THP-1 monocytes and prevents lipopolysaccharide (LPS)-induced growth effects on those cell types. Finally, PRS CK STORM prevents LPS-induced TNF-A and IL-1Β secretion from PBMC and from THP-1 cells at the same level as hydrocortisone, demonstrating its anti-inflammatory potency.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Mesenchymal Stem Cells / COVID-19 Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Biom12040534

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Mesenchymal Stem Cells / COVID-19 Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Biom12040534