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[Application of oral fluid in SARS-CoV-2 nucleic acid and antibody detection].
Huang, Q; Liu, F Y; Mao, N Y; Sun, J Y; Dong, M; Xie, H; Liu, F; Zhang, H; Yu, X L; Dong, J P; Xu, Wenbo; Huang, Fang.
  • Huang Q; School of Public Health, Capital Medical University, Beijing 100069, China Institute of Preventive Immunization, Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Beijing 100013, China.
  • Liu FY; Department of Microbiology Laboratory, Beijing Haidian Center for Disease Control and Prevention, Beijing 100094, China.
  • Mao NY; Institute for Viral Disease Control and Prevention, Chinese Centers for Disease control and Prevention, Beijing 102206, China NHC Key Laboratory for Medical Virology and Viral Diseases, Beijing 102206, China.
  • Sun JY; Department Infectious Disease, Beijing Haidian Hospital, Beijing 100080, China.
  • Dong M; Institute of Preventive Immunization, Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Beijing 100013, China.
  • Xie H; Institute of Preventive Immunization, Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Beijing 100013, China.
  • Liu F; Department of Microbiology Laboratory, Beijing Haidian Center for Disease Control and Prevention, Beijing 100094, China.
  • Zhang H; Department of Microbiology Laboratory, Beijing Haidian Center for Disease Control and Prevention, Beijing 100094, China.
  • Yu XL; Institute of Preventive Immunization, Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Beijing 100013, China.
  • Dong JP; Department Infectious Disease, Beijing Haidian Hospital, Beijing 100080, China.
  • Xu W; Institute for Viral Disease Control and Prevention, Chinese Centers for Disease control and Prevention, Beijing 102206, China NHC Key Laboratory for Medical Virology and Viral Diseases, Beijing 102206, China.
  • Huang F; School of Public Health, Capital Medical University, Beijing 100069, China Institute of Preventive Immunization, Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Beijing 100013, China.
Zhonghua Yu Fang Yi Xue Za Zhi ; 56(3): 355-359, 2022 Mar 06.
Article in Chinese | MEDLINE | ID: covidwho-1776391
ABSTRACT
This study aimed to explore the application value of new biological specimen oral fluid in SARS-CoV-2 nucleic acid and antibody detection. Oral fluid and paired respiratory and blood specimens from 7 confirmed cases of two COVID-19 cluster epidemic were collected in Beijing from October to November 2021. SARS-CoV-2 virus and IgG antibody were detected by real time PCR kits and serum antibody detection reagents, and SARS-CoV-2 IgG antibody in oral fluids was detected by a new established method of magnetic particle chemiluminescence. The results showed that the nucleic acid amplification test of SARS-CoV-2 on nasopharyngeal swabs, throat swabs and oral fluid specimens from 3 confirmed cases of COVID-19 was positive, among which the Ct value for ORF1a/b and N gene of oral fluid samples in 2 cases was close to that of throat swab, and the Ct value of oral fluid sample for 1 case was higher than that of throat swab. The complete genome sequence of one oral fluid specimen was obtained, which belonged to the VOC/Delta variant strain. The SARS-CoV-2 IgG antibodies of the paired oral fluid and serum were all positive, and the S/CO values of oral fluid were all lower than those of serum. The series of oral fluid results showed that SARS-CoV-2 IgG antibody level increased from 11 to 32 days after the onset of the disease.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: Chinese Journal: Zhonghua Yu Fang Yi Xue Za Zhi Year: 2022 Document Type: Article Affiliation country: Cma.j.cn112150-20211211-01146

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: Chinese Journal: Zhonghua Yu Fang Yi Xue Za Zhi Year: 2022 Document Type: Article Affiliation country: Cma.j.cn112150-20211211-01146