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Next-generation sequencing: A new avenue to understand viral RNA-protein interactions.
Zhou, Yiyang; Sotcheff, Stephanea L; Routh, Andrew L.
  • Zhou Y; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas, USA. Electronic address: yizhou@utmb.edu.
  • Sotcheff SL; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas, USA.
  • Routh AL; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas, USA; Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch, Galveston, Texas, USA; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA.
J Biol Chem ; 298(5): 101924, 2022 05.
Article in English | MEDLINE | ID: covidwho-1778266
ABSTRACT
The genomes of RNA viruses present an astonishing source of both sequence and structural diversity. From intracellular viral RNA-host interfaces to interactions between the RNA genome and structural proteins in virus particles themselves, almost the entire viral lifecycle is accompanied by a myriad of RNA-protein interactions that are required to fulfill their replicative potential. It is therefore important to characterize such rich and dynamic collections of viral RNA-protein interactions to understand virus evolution and their adaptation to their hosts and environment. Recent advances in next-generation sequencing technologies have allowed the characterization of viral RNA-protein interactions, including both transient and conserved interactions, where molecular and structural approaches have fallen short. In this review, we will provide a methodological overview of the high-throughput techniques used to study viral RNA-protein interactions, their biochemical mechanisms, and how they evolved from classical methods as well as one another. We will discuss how different techniques have fueled virus research to characterize how viral RNA and proteins interact, both locally and on a global scale. Finally, we will present examples on how these techniques influence the studies of clinically important pathogens such as HIV-1 and SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Proteins / High-Throughput Nucleotide Sequencing Type of study: Prognostic study / Reviews Limits: Humans Language: English Journal: J Biol Chem Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Proteins / High-Throughput Nucleotide Sequencing Type of study: Prognostic study / Reviews Limits: Humans Language: English Journal: J Biol Chem Year: 2022 Document Type: Article