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Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein.
Silva, Patricia Bento da; Silva, Jaqueline Rodrigues da; Rodrigues, Mosar Corrêa; Vieira, Julia Augusto; Andrade, Ikaro Alves de; Nagata, Tatsuya; Santos, Alexandre Silva; Silva, Sebastião William da; Rocha, Marcia Cristina Oliveira da; Báo, Sônia Nair; Moraes-Vieira, Pedro M; Proença-Modena, José; Angelim, Monara K C; de Souza, Gabriela Fabiano; Muraro, Stefanie Primon; de Barros, André Luis Branco; de Souza Martins, Glêndara Aparecida; Ribeiro-Dias, Fátima; Machado, Giovanna; Fessel, Melissa Regina; Chudzinski-Tavassi, Ana Marisa; Ronconi, Célia Machado; Gonçalves, Debora; Curi, Rui; Oliveira, Osvaldo N; Azevedo, Ricardo Bentes.
  • Silva PBD; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Silva JRD; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Rodrigues MC; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Vieira JA; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Andrade IA; Virology and Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Nagata T; Virology and Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Santos AS; Optical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Silva SWD; Optical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
  • Rocha MCOD; Electron Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasília, Distrito Federal, 70910-900, Brazil.
  • Báo SN; Electron Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasília, Distrito Federal, 70910-900, Brazil.
  • Moraes-Vieira PM; Department of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
  • Proença-Modena J; Department of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
  • Angelim MKC; Department of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
  • de Souza GF; Department of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
  • Muraro SP; Department of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
  • de Barros ALB; Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
  • de Souza Martins GA; Programa de Pós-graduação em Ciência e Tecnologia de Alimentos, Federal University of Tocantins, 77001-090, Brazil.
  • Ribeiro-Dias F; Laboratório de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil.
  • Machado G; Centro de Tecnologias Estratégicas do Nordeste, Av. Prof. Luiz Freire 01, Recife, Pernambuco, 50740-540, Brazil.
  • Fessel MR; Bioindustrial Center, Butantan Institute, Sao Paulo, São Paulo, 05503-900, Brazil.
  • Chudzinski-Tavassi AM; Bioindustrial Center, Butantan Institute, Sao Paulo, São Paulo, 05503-900, Brazil.
  • Ronconi CM; Departamento de Química Inorgânica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro de São João Batista, s/n, 24020-141, Niterói, RJ, Brazil.
  • Gonçalves D; Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, São Paulo, 13560-970, Brazil.
  • Curi R; Bioindustrial Center, Butantan Institute, Sao Paulo, São Paulo, 05503-900, Brazil; Interdisciplinary Program of Health Sciences, Cruzeiro do Sul University, Sao Paulo, 01311-925, Brazil.
  • Oliveira ON; Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, São Paulo, 13560-970, Brazil. Electronic address: chu@ifsc.usp.br.
  • Azevedo RB; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil. Electronic address: razevedo@unb.br.
Talanta ; 243: 123355, 2022 Jun 01.
Article in English | MEDLINE | ID: covidwho-1778463
ABSTRACT
Mass testing for the diagnosis of COVID-19 has been hampered in many countries owing to the high cost of genetic material detection. This study reports on a low-cost immunoassay for detecting SARS-CoV-2 within 30 min using dynamic light scattering (DLS). The immunosensor comprises 50-nm gold nanoparticles (AuNPs) functionalized with antibodies against SARS-CoV-2 spike glycoprotein, whose bioconjugation was confirmed using transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and surface-enhanced Raman scattering spectroscopy (SERS). The specific binding of the bioconjugates to the spike protein led to an increase in bioconjugate size, with a limit of detection (LOD) 5.29 × 103 TCID50/mL (Tissue Culture Infectious Dose). The immunosensor was also proven to be selective upon interaction with influenza viruses once no increase in size was observed after DLS measurement. The strategy proposed here aimed to use antibodies conjugated to AuNPs as a generic platform that can be extended to other detection principles, enabling technologies for low-cost mass testing for COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Metal Nanoparticles / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Talanta Year: 2022 Document Type: Article Affiliation country: J.talanta.2022.123355

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Metal Nanoparticles / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Talanta Year: 2022 Document Type: Article Affiliation country: J.talanta.2022.123355