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A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2.
Sarkar, Anindya; Paul, Sathi; Singh, Charandeep; Chowdhury, Nilkanta; Nag, Papri; Das, Swarnava; Kumar, Sahil; Sharma, Anshul; Das, Deepjyoti Kumar; Dutta, Dipak; Thakur, Krishan Gopal; Bagchi, Angshuman; Shriti, Surbhi; Das, Kali P; Ringe, Rajesh P; Das, Sampa.
  • Sarkar A; Division of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
  • Paul S; Division of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
  • Singh C; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
  • Chowdhury N; Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, 741235, India.
  • Nag P; Division of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
  • Das S; Division of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
  • Kumar S; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
  • Sharma A; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
  • Das DK; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
  • Dutta D; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
  • Thakur KG; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
  • Bagchi A; Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, 741235, India.
  • Shriti S; Division of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
  • Das KP; Formerly, Department of Chemistry, Bose Institute, 93/1 A.P.C. Road, Kolkata, 700009, India.
  • Ringe RP; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
  • Das S; Division of Plant Biology, Bose Institute, P/12 C.I.T. Scheme VII(M), Kolkata, 700054, India.
Virus Res ; 315: 198768, 2022 07 02.
Article in English | MEDLINE | ID: covidwho-1778498
ABSTRACT
COVID-19 caused by SARS-CoV-2 virus has had profound impact on the world in the past two years. Intense research is going on to find effective drugs to combat the disease. Over the past year several vaccines were approved for immunization. But SARS-CoV-2 being an RNA virus is continuously mutating to generate new variants, some of which develop features of immune escape. This raised serious doubts over the long-term efficacy of the vaccines. We have identified a unique mannose binding plant lectin from Narcissus tazetta bulb, NTL-125, which effectively inhibits SARS-CoV-2 replication in Vero-E6 cell line. In silico docking studies revealed that NTL-125 has strong affinity to viral Spike RBD protein, preventing it from attaching to hACE2 receptor, the gateway to cellular entry. Binding analyses revealed that all the mutant variants of Spike protein also have stronger affinity for NTL-125 than hACE2. The unique α-helical tail of NTL-125 plays most important role in binding to RBD of Spike. NTL-125 also interacts effectively with some glycan moieties of S-protein in addition to amino acid residues adding to the binding strength. Thus, NTL-125 is a highly potential antiviral compound of natural origin against SARS-CoV-2 and may serve as an important therapeutic for management of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plant Lectins / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / SARS-CoV-2 Topics: Vaccines / Variants Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2022 Document Type: Article Affiliation country: J.virusres.2022.198768

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plant Lectins / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / SARS-CoV-2 Topics: Vaccines / Variants Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2022 Document Type: Article Affiliation country: J.virusres.2022.198768