Association between olfactory pathway gene variants and obesity in Chinese Han population: A case-control study based on genetic score.
Gene
; 825: 146442, 2022 May 30.
Article
in English
| MEDLINE | ID: covidwho-1783331
ABSTRACT
BACKGROUND:
Our previous bivariate genome-wide association study in dizygotic twins suggested that the olfactory transduction pathway genes were associated with obesity in Northern Han Chinese adults. In this study, we attempted to verify the associations of the olfactory transduction pathway genes score with obesity in population with the same genetic background, and to estimate the interaction between gene variants and potential environment factors.METHODS:
A case-control study was conducted in Qingdao, China in 2019-2021, which enrolled 301 obesity cases and 307 controls. Based on the candidate gene selection method, 29 single nucleotide polymorphisms (SNPs) in 7 olfactory pathway genes were selected. Genomic deoxyribonucleic acid (DNA) was isolated and purified from the peripheral blood leukocytes by using DNA extraction kits and was genotyped by the MassArray system. The weighted genetic score of each gene was calculated to analyze the effect of whole gene. The effect of gene scores on obesity and the gene-environment interaction were estimated by logistic regression.RESULTS:
After adjusting for age, sex, smoking, alcohol drinking, physical activity, we observed positive associations of OR4D1 (OR = 1.531, 95% CI = 1.083-2.164, P = 0.016) and OR52K1 (OR = 1.437, 95% CI = 1.055-1.957, P = 0.022) gene scores with obesity, as well as negative associations of OR2L8 (OR = 0.708, 95% CI = 0.504-0.995, P = 0.046) and CALML3 (OR = 0.601, 95% CI = 0.410-0.881, P = 0.009) gene scores with obesity. Significant multiplicative model interaction between OR4D1 and smoking (Pinteraction = 0.041) as well as CALML3 and smoking (Pinteraction = 0.026) on obesity were identified. Stratified analysis showed that in smokers, OR4D1 gene score was positively associated with obesity (OR = 2.673, 95% CI = 1.348-5.299, P = 0.005) and CALML3 gene score was negatively correlated with obesity (OR = 0.252, 95% CI = 0.103-0.618, P = 0.003). The relationships were not statistically significant in non-smokers (OR4D1 OR = 1.216, 95% CI = 0.806-1.836, P = 0.351; CALML3 OR = 0.764, 95% CI = 0.492-1.188, P = 0.232).CONCLUSIONS:
Genetic variations in the olfactory pathway were associated with obesity in Northern Han Chinese adults. Smoking modified the effect of OR4D1 and CALML3 gene variants on obesity.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Olfactory Pathways
/
Genome-Wide Association Study
Type of study:
Observational study
/
Prognostic study
Topics:
Variants
Limits:
Adult
/
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Gene
Year:
2022
Document Type:
Article
Affiliation country:
J.gene.2022.146442
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