Porcine intraepithelial lymphocytes undergo migration and produce an antiviral response following intestinal virus infection.
Commun Biol
; 5(1): 252, 2022 03 22.
Article
in English
| MEDLINE | ID: covidwho-1788322
ABSTRACT
The location of intraepithelial lymphocytes (IELs) between epithelial cells provide a first line of immune defense against enteric infection. It is assumed that IELs migrate only along the basement membrane or into the lateral intercellular space (LIS) between epithelial cells. Here, we identify a unique transepithelial migration of porcine IELs as they move to the free surface of the intestinal epithelia. The major causative agent of neonatal diarrhea in piglets, porcine epidemic diarrhea virus (PEDV), increases the number of IELs entering the LIS and free surface of the intestinal epithelia, driven by chemokine CCL2 secreted from virus-infected intestinal epithelial cells. Remarkably, only virus pre-activated IELs inhibits PEDV infection and their antiviral activity depends on the further activation by virus-infected cells. Although high levels of perforin is detected in the co-culture system, the antiviral function of activated IELs is mainly mediated by IFN-γ secretion inducing robust antiviral response in virus-infected cells. Our results uncover a unique migratory behavior of porcine IELs as well as their protective role in the defense against intestinal infection.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Virus Diseases
/
Coronavirus Infections
/
Porcine epidemic diarrhea virus
/
Intraepithelial Lymphocytes
/
Intestinal Diseases
Limits:
Animals
Language:
English
Journal:
Commun Biol
Year:
2022
Document Type:
Article
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