Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA.
J Immunol Methods
; 505: 113275, 2022 06.
Article
in English
| MEDLINE | ID: covidwho-1796490
ABSTRACT
Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
SARS-CoV-2
/
COVID-19
Topics:
Vaccines
Limits:
Animals
Language:
English
Journal:
J Immunol Methods
Year:
2022
Document Type:
Article
Affiliation country:
J.jim.2022.113275
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