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Molecularly imprinted polypyrrole based sensor for the detection of SARS-CoV-2 spike glycoprotein.
Ratautaite, Vilma; Boguzaite, Raimonda; Brazys, Ernestas; Ramanaviciene, Almira; Ciplys, Evaldas; Juozapaitis, Mindaugas; Slibinskas, Rimantas; Bechelany, Mikhael; Ramanavicius, Arunas.
  • Ratautaite V; Laboratory of Nanotechnology, Department of Functional Materials and Electronics, Center for Physical Sciences and Technology, Sauletekio av. 3, Vilnius LT-10257, Lithuania.
  • Boguzaite R; NanoTechnas - Center of Nanotechnology and Materials Science at Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, LT-03225, Vilnius, Lithuania.
  • Brazys E; Laboratory of Nanotechnology, Department of Functional Materials and Electronics, Center for Physical Sciences and Technology, Sauletekio av. 3, Vilnius LT-10257, Lithuania.
  • Ramanaviciene A; NanoTechnas - Center of Nanotechnology and Materials Science at Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, LT-03225, Vilnius, Lithuania.
  • Ciplys E; NanoTechnas - Center of Nanotechnology and Materials Science at Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, LT-03225, Vilnius, Lithuania.
  • Juozapaitis M; NanoTechnas - Center of Nanotechnology and Materials Science at Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, LT-03225, Vilnius, Lithuania.
  • Slibinskas R; NanoTechnas - Center of Nanotechnology and Materials Science at Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, LT-03225, Vilnius, Lithuania.
  • Bechelany M; Institute of Biotechnology, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, Lithuania.
  • Ramanavicius A; NanoTechnas - Center of Nanotechnology and Materials Science at Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, LT-03225, Vilnius, Lithuania.
Electrochim Acta ; 403: 139581, 2022 Jan 20.
Article in English | MEDLINE | ID: covidwho-1796883
ABSTRACT
This study describes the application of a polypyrrole-based sensor for the determination of SARS-CoV-2-S spike glycoprotein. The SARS-CoV-2-S spike glycoprotein is a spike protein of the coronavirus SARS-CoV-2 that recently caused the worldwide spread of COVID-19 disease. This study is dedicated to the development of an electrochemical determination method based on the application of molecularly imprinted polymer technology. The electrochemical sensor was designed by molecular imprinting of polypyrrole (Ppy) with SARS-CoV-2-S spike glycoprotein (MIP-Ppy). The electrochemical sensors with MIP-Ppy and with polypyrrole without imprints (NIP-Ppy) layers were electrochemically deposited on a platinum electrode surface by a sequence of potential pulses. The performance of polymer layers was evaluated by pulsed amperometric detection. According to the obtained results, a sensor based on MIP-Ppy is more sensitive to the SARS-CoV-2-S spike glycoprotein than a sensor based on NIP-Ppy. Also, the results demonstrate that the MIP-Ppy layer is more selectively interacting with SARS-CoV-2-S glycoprotein than with bovine serum albumin. This proves that molecularly imprinted MIP-Ppy-based sensors can be applied for the detection of SARS-CoV-2 virus proteins.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies Language: English Journal: Electrochim Acta Year: 2022 Document Type: Article Affiliation country: J.electacta.2021.139581

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies Language: English Journal: Electrochim Acta Year: 2022 Document Type: Article Affiliation country: J.electacta.2021.139581