Simultaneous Detection of Multiple Respiratory Viruses Among SARS-CoV-2-Positive and Negative Patients by Multiplex TaqMan One-Step Real-Time PCR
Jundishapur Journal of Microbiology
; 15(1), 2022.
Article
in English
| EMBASE | ID: covidwho-1798771
ABSTRACT
Background:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has clinical manifestations similar to other common respiratory viral infections. There are limited data on the frequency of viral respiratory coinfection among patients infected with SARS-CoV-2 in Iran.Objectives:
This cross-sectional study investigated the prevalence of multiple respiratory viruses among SARS-CoV-2-positive and negative patients during the coronavirus disease 2019 (COVID-19) pandemic in Iran.Methods:
We included oropharyngeal/nasopharyngeal swab specimens of patients suspected of COVID-19 from December 2020 to March 2021. A reliable multiplex TaqMan one-step real-time PCR method was employed to detect 17 viral respiratory pathogens si-multaneously. Descriptive analyses were performed to characterize the specimens concerning age, gender, clinical manifestations, and underlying disease.Results:
Multiple respiratory viruses with a frequency of 18.78% were detected in 197 studied patients. Human metapneumovirus was the most prevalent pathogen detected in both SARS-CoV-2-positive (n = 7, 7.7%) and negative (n = 7, 6.6%) patients. Moreover, the frequency rate of viral infection was almost the same in both SARS-CoV-2-positive (18.68%) and negative (18.86%) patients. Altogether, there were no differences in baseline demographic characteristics such as age, sex, clinical symptoms, and comorbidities between the two groups (P > 0.05).Conclusions:
The data presented here expand our understanding of the epidemiology of multiple types of viral respiratory pathogens in suspected COVID-19 patients. Therefore, simultaneous screening of other viral respiratory pathogens will be helpful for clinicians and researchers interested in the control of viral respiratory tract infections.
adult; article; coinfection; comorbidity; controlled study; coronavirus disease 2019; cross-sectional study; demographics; female; gender; human; Human metapneumovirus; human tissue; infectious agent; major clinical study; male; nasopharyngeal swab; nonhuman; real time polymerase chain reaction; respiratory tract infection; respiratory virus; Severe acute respiratory syndrome coronavirus 2; viral respiratory tract infection; virology
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Jundishapur Journal of Microbiology
Year:
2022
Document Type:
Article
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