Towards an Electrochemical Immunosensor for the Detection of Antibodies against SARS-CoV-2 Spike Protein
Journal of the Electrochemical Society
; 169(3):7, 2022.
Article
in English
| Web of Science | ID: covidwho-1799211
ABSTRACT
Herein we report the electrochemical system for the detection of specific antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins in blood serum patient samples after coronavirus disease 2019 (COVID-19). For this purpose, the recombinant SARS-CoV-2 spike protein (SCoV2-rS) was covalently immobilised on the surface of the gold electrode pre-modified with mixed self-assembled monolayer (SAMmix) consisting of 11-mercaptoundecanoic acid and 6-mercapto-1-hexanol. The affinity interaction of SCoV2-rS with specific antibodies against this protein (anti-rS) was detected using two electrochemical methods:
cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The anti-rS was detected with a detection limit of 2.53 nM and 1.99 nM using CV and EIS methods, respectively. The developed electrochemical immunosensor is suitable for the confirmation of COVID-19 infection or immune response in humans after vaccination.
Full text:
Available
Collection:
Databases of international organizations
Database:
Web of Science
Language:
English
Journal:
Journal of the Electrochemical Society
Year:
2022
Document Type:
Article
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