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Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids.
Eriksen, Anne Zebitz; Møller, Rasmus; Makovoz, Bar; tenOever, Benjamin R; Blenkinsop, Timothy A.
  • Eriksen AZ; Department of Cell, Development and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • Møller R; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • Makovoz B; Department of Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • tenOever BR; Department of Health Technology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
  • Blenkinsop TA; Department of Microbiology, New York University, New York, NY 10016, USA.
STAR Protoc ; 3(2): 101383, 2022 06 17.
Article in English | MEDLINE | ID: covidwho-1799657
ABSTRACT
Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies Limits: Adult / Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2022.101383

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies Limits: Adult / Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2022.101383