Assessing Molecular Regulation of Vascular Permeability Using a VEGF-Inducible Zebrafish Model.
Methods Mol Biol
; 2475: 339-350, 2022.
Article
in English
| MEDLINE | ID: covidwho-1802606
ABSTRACT
Vascular endothelial growth factor (VEGF) stimulates vascular permeability in a variety of human pathologies, such as cancer, ischemic stroke, cardiovascular disease, retinal conditions, and COVID-19-associated pulmonary edema, sepsis, acute lung injury, and acute respiratory distress syndrome. Comprehensive investigation of the molecular mechanisms of VEGF-induced vascular permeability has been hindered by the lack of in vivo models that easily facilitate genetic manipulation studies in real time. To address this need, we generated a heat-inducible VEGF transgenic zebrafish model of vascular permeability. Here, we describe how this zebrafish model can be used to monitor VEGF-induced vascular permeability through live in vivo imaging to identify genetic regulators that play key roles in vascular barrier integrity in physiological conditions and human disease processes.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Capillary Permeability
/
COVID-19
Limits:
Animals
Language:
English
Journal:
Methods Mol Biol
Journal subject:
Molecular Biology
Year:
2022
Document Type:
Article
Affiliation country:
978-1-0716-2217-9_25
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