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A highly photostable and bright green fluorescent protein.
Hirano, Masahiko; Ando, Ryoko; Shimozono, Satoshi; Sugiyama, Mayu; Takeda, Noriyo; Kurokawa, Hiroshi; Deguchi, Ryusaku; Endo, Kazuki; Haga, Kei; Takai-Todaka, Reiko; Inaura, Shunsuke; Matsumura, Yuta; Hama, Hiroshi; Okada, Yasushi; Fujiwara, Takahiro; Morimoto, Takuya; Katayama, Kazuhiko; Miyawaki, Atsushi.
  • Hirano M; Biotechnological Optics Research Team, RIKEN Center for Advanced Photonics, Saitama, Japan.
  • Ando R; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, Saitama, Japan.
  • Shimozono S; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, Saitama, Japan.
  • Sugiyama M; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, Saitama, Japan.
  • Takeda N; Asamushi Research Center for Marine Biology, Tohoku University, Aomori, Japan.
  • Kurokawa H; Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan.
  • Deguchi R; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, Saitama, Japan.
  • Endo K; Department of Biology, Miyagi University of Education, Sendai, Japan.
  • Haga K; Department of Biology, Miyagi University of Education, Sendai, Japan.
  • Takai-Todaka R; Narita Elementary School, Miyagi, Japan.
  • Inaura S; Department of Infection Control and Immunology, Omura Satoshi Memorial Institute, Kitasato University, Tokyo, Japan.
  • Matsumura Y; Department of Infection Control and Immunology, Omura Satoshi Memorial Institute, Kitasato University, Tokyo, Japan.
  • Hama H; Safety Science Laboratories, Kao Corporation, Tokyo, Japan.
  • Okada Y; Safety Science Laboratories, Kao Corporation, Tokyo, Japan.
  • Fujiwara T; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, Saitama, Japan.
  • Morimoto T; Laboratory for Cell Polarity Regulation, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.
  • Katayama K; Department of Cell Biology and Department of Physics, UBI and WPI-IRCN, The University of Tokyo, Tokyo, Japan.
  • Miyawaki A; Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.
Nat Biotechnol ; 40(7): 1132-1142, 2022 07.
Article in English | MEDLINE | ID: covidwho-1805637
ABSTRACT
The low photostability of fluorescent proteins is a limiting factor in many applications of fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae. StayGold is over one order of magnitude more photostable than any currently available fluorescent protein and has a cellular brightness similar to mNeonGreen. We used StayGold to image the dynamics of the endoplasmic reticulum (ER) with high spatiotemporal resolution over several minutes using structured illumination microscopy (SIM) and observed substantially less photobleaching than with a GFP variant optimized for stability in the ER. Using StayGold fusions and SIM, we also imaged the dynamics of mitochondrial fusion and fission and mapped the viral spike proteins in fixed cells infected with severe acute respiratory syndrome coronavirus 2. As StayGold is a dimer, we created a tandem dimer version that allowed us to observe the dynamics of microtubules and the excitatory post-synaptic density in neurons. StayGold will substantially reduce the limitations imposed by photobleaching, especially in live cell or volumetric imaging.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Topics: Variants Limits: Humans Language: English Journal: Nat Biotechnol Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: S41587-022-01278-2

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Topics: Variants Limits: Humans Language: English Journal: Nat Biotechnol Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: S41587-022-01278-2