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Surveillance and Correlation of Severe Acute Respiratory Syndrome Coronavirus 2 Viral RNA, Antigen, Virus Isolation, and Self-Reported Symptoms in a Longitudinal Study With Daily Sampling.
Bonenfant, Gaston; Deyoe, Jessica E; Wong, Terianne; Grijalva, Carlos G; Cui, Dan; Talbot, H Keipp; Hassell, Norman; Halasa, Natasha; Chappell, James; Thornburg, Natalie J; Rolfes, Melissa A; Wentworth, David E; Zhou, Bin.
  • Bonenfant G; COVID-19 Emergency Response, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Deyoe JE; Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA.
  • Wong T; COVID-19 Emergency Response, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Grijalva CG; Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA.
  • Cui D; COVID-19 Emergency Response, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Talbot HK; Vanderbilt University Medical Center, Nashville, Tennessee, USA.
  • Hassell N; COVID-19 Emergency Response, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Halasa N; General Dynamics Information Technology, Inc, Falls Church, Virginia, USA.
  • Chappell J; Vanderbilt University Medical Center, Nashville, Tennessee, USA.
  • Thornburg NJ; COVID-19 Emergency Response, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Rolfes MA; Vanderbilt University Medical Center, Nashville, Tennessee, USA.
  • Wentworth DE; Vanderbilt University Medical Center, Nashville, Tennessee, USA.
  • Zhou B; COVID-19 Emergency Response, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Clin Infect Dis ; 75(10): 1698-1705, 2022 Nov 14.
Article in English | MEDLINE | ID: covidwho-2116480
ABSTRACT
The novel coronavirus pandemic incited unprecedented demand for assays that detect viral nucleic acids, viral proteins, and corresponding antibodies. The 320 molecular diagnostics in receipt of US Food and Drug Administration emergency use authorization mainly focus on viral detection; however, no currently approved test can be used to infer infectiousness, that is, the presence of replicable virus. As the number of tests conducted increased, persistent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA positivity by reverse-transcription polymerase chain reaction (RT-PCR) in some individuals led to concerns over quarantine guidelines. To this end, we attempted to design an assay that reduces the frequency of positive test results from individuals who do not shed culturable virus. We describe multiplex quantitative RT-PCR assays that detect genomic RNA (gRNA) and subgenomic RNA (sgRNA) species of SARS-CoV-2, including spike, nucleocapsid, membrane, envelope, and ORF8. Viral RNA abundances calculated from these assays were compared with antigen presence, self-reported symptoms, and culture outcome (virus isolation) using samples from a 14-day longitudinal household transmission study. By characterizing the clinical and molecular dynamics of infection, we show that sgRNA detection has higher predictive value for culture outcome compared to detection of gRNA alone. Our findings suggest that sgRNA presence correlates with active infection and may help identify individuals shedding culturable virus.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study / Qualitative research Topics: Long Covid Limits: Humans Language: English Journal: Clin Infect Dis Journal subject: Communicable Diseases Year: 2022 Document Type: Article Affiliation country: Cid

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study / Qualitative research Topics: Long Covid Limits: Humans Language: English Journal: Clin Infect Dis Journal subject: Communicable Diseases Year: 2022 Document Type: Article Affiliation country: Cid