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SARS-CoV-2 productively infects primary human immune system cells in vitro and in COVID-19 patients.
Pontelli, Marjorie C; Castro, Ítalo A; Martins, Ronaldo B; La Serra, Leonardo; Veras, Flávio P; Nascimento, Daniele C; Silva, Camila M; Cardoso, Ricardo S; Rosales, Roberta; Gomes, Rogério; Lima, Thais M; Souza, Juliano P; Vitti, Brenda C; Caetité, Diego B; de Lima, Mikhael H F; Stumpf, Spencer D; Thompson, Cassandra E; Bloyet, Louis-Marie; Toller-Kawahisa, Juliana E; Giannini, Marcela C; Bonjorno, Letícia P; Lopes, Maria I F; Batah, Sabrina S; Siyuan, Li; Luppino-Assad, Rodrigo; Almeida, Sergio C L; Oliveira, Fabiola R; Benatti, Maíra N; Pontes, Lorena L F; Santana, Rodrigo C; Vilar, Fernando C; Auxiliadora-Martins, Maria; Shi, Pei-Yong; Cunha, Thiago M; Calado, Rodrigo T; Alves-Filho, José C; Zamboni, Dario S; Fabro, Alexandre T; Louzada-Junior, Paulo; Oliveira, Rene D R; Whelan, Sean P J; Cunha, Fernando Q; Arruda, Eurico.
  • Pontelli MC; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Castro ÍA; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Martins RB; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • La Serra L; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Veras FP; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Nascimento DC; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Silva CM; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Cardoso RS; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Rosales R; Department of Cell and Molecular Biology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Gomes R; Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Lima TM; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Souza JP; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Vitti BC; Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Caetité DB; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • de Lima MHF; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Stumpf SD; Department of Biochemistry & Molecular Biology, the University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Thompson CE; Department of Biochemistry & Molecular Biology, the University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Bloyet LM; Department of Biochemistry & Molecular Biology, the University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Toller-Kawahisa JE; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Giannini MC; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Bonjorno LP; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Lopes MIF; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Batah SS; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Siyuan L; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Luppino-Assad R; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Almeida SCL; Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Oliveira FR; Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Benatti MN; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Pontes LLF; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Santana RC; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Vilar FC; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Auxiliadora-Martins M; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Shi PY; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Cunha TM; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Calado RT; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Alves-Filho JC; Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Zamboni DS; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Fabro AT; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Louzada-Junior P; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Oliveira RDR; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Whelan SPJ; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Cunha FQ; Divisions of Clinical Immunology, Infectious Diseases and Intensive Care Unit, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Arruda E; Department of Biochemistry & Molecular Biology, the University of Texas Medical Branch, Galveston, TX 77555, USA.
J Mol Cell Biol ; 14(4)2022 08 17.
Article in English | MEDLINE | ID: covidwho-1806451
ABSTRACT
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with a hyperinflammatory state and lymphocytopenia, a hallmark that appears as both signature and prognosis of disease severity outcome. Although cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion, it is still unclear whether direct SARS-CoV-2 infection of immune cells could also play a role in this scenario by harboring viral replication. We found that monocytes, as well as both B and T lymphocytes, were susceptible to SARS-CoV-2 infection in vitro, accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive T cell apoptosis. In addition, flow cytometry and immunofluorescence analysis revealed that SARS-CoV-2 was frequently detected in monocytes and B lymphocytes from coronavirus disease 2019 (COVID-19) patients. The rates of SARS-CoV-2-infected monocytes in peripheral blood mononuclear cells from COVID-19 patients increased over time from symptom onset, with SARS-CoV-2-positive monocytes, B cells, and CD4+ T lymphocytes also detected in postmortem lung tissue. These results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in COVID-19 patients might have important implications for disease pathogenesis and progression, immune dysfunction, and virus spread within the host.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: Jmcb

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: Jmcb