Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip.
BMC Vet Res
; 18(1): 140, 2022 Apr 18.
Article
in English
| MEDLINE | ID: covidwho-1808371
ABSTRACT
BACKGROUND:
Porcine epidemic diarrhea virus (PEDV) is one of the most important enteric viruses causing diarrhea in pigs. The establishment of a rapid detection method applicable in field conditions will be conducive to early detection of pathogen and implementation of relevant treatment. A novel nucleic acid amplification method, recombinase polymerase amplification (RPA), has been widely used for infectious disease diagnosis.RESULTS:
In the present study, a reverse transcription (RT)-RPA assay combined with lateral flow dipstrip (LFD) was established for the visual detection of PEDV by targeting the N gene. The RT-RPA-LFD assay detected as low as 102 copies/µL of PEDV genomic RNA standard. Moreover, the novel RT-RPA-LFD assay did not show cross-reactivity with common swine pathogens, demonstrating high specificity. The performance of the assay for detection of clinical samples was also evaluated. A total number of 86 clinical samples were tested by RT-RPA-LFD and RT-PCR. The detection results of RT-RPA-LFD were compared with those of RT-PCR, with a coincidence rate of 96.5%.CONCLUSION:
The newly established RT-RPA-LFD assay in our study had high sensitivity and specificity, with a potential to use in resource-limited areas and countries.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Porcine epidemic diarrhea virus
Type of study:
Diagnostic study
/
Experimental Studies
/
Prognostic study
/
Randomized controlled trials
Limits:
Animals
Language:
English
Journal:
BMC Vet Res
Journal subject:
Veterinary Medicine
Year:
2022
Document Type:
Article
Affiliation country:
S12917-022-03232-5
Similar
MEDLINE
...
LILACS
LIS