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A Label-Free Electrochemical Impedimetric Immunosensor with Biotinylated-Antibody for SARS-CoV-2 Nucleoprotein Detection in Saliva.
Wu, Ching-Chou; Chiang, Yu-Huan; Chiang, Hsin-Yu.
  • Wu CC; Department of Bio-industrial Mechatronics Engineering, National Chung Hsing University, Taichung 402, Taiwan.
  • Chiang YH; Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung 402, Taiwan.
  • Chiang HY; Department of Bio-industrial Mechatronics Engineering, National Chung Hsing University, Taichung 402, Taiwan.
Biosensors (Basel) ; 12(5)2022 Apr 22.
Article in English | MEDLINE | ID: covidwho-1809707
ABSTRACT
The timely detecting of SARS-CoV-2 coronavirus antigens for infection validation is an urgent request for COVID-19 pandemic control. This study constructed label-free electrochemical impedance spectroscopy (EIS)-based immunosensors based on gold nanostructured screen-printed carbon electrodes (AuNS/SPCEs) to detect the SARS-CoV-2 nucleocapsid protein (N-protein) in saliva. Using short-chain 3-mercaptopropionic acid (MPA) as a linker to covalently bond streptavidin (SA) and bovine serum albumin (BSA) for controlling the oriented immobilization of the biotinylated anti-N-protein antibody (BioAb) can offer a greater sensitivity, a lower limit of detection (LOD), and better reproducibility of immunosensors (defined as BioAb/SA-BSA/MPA/AuNS/SPCEs) than the antibody randomly immobilized immunosensors and the long-chain 11-mercaptoundecanoic acid (MUA)-modified immunosensors (BioAb/SA-BSA/MUA/AuNS/SPCEs). The BioAb/SA-BSA/MPA/AuNS/SPCE-based immunosensors presented good linearity from 0.01 ng/mL to 100 ng/mL and a low LOD of 6 pg/mL in a phosphate buffer solution (PBS) and PBS-diluted saliva. Moreover, the immunosensor exhibited little cross-activity with other viral antigens such as MERS-CoV N-protein, influenza A N-protein, influenza B N-protein, and SARS-CoV-2 spike protein, indicating the high specificity of the immunosensors. The disposable label-free EIS-based immunosensors have promising potential in facilitating the rapid and sensitive tests of saliva-based COVID-19 diagnostics.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Influenza, Human / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Topics: Long Covid Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12050265

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Influenza, Human / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Topics: Long Covid Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12050265