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Mouthrinses against SARS-CoV-2 - High antiviral effectivity by membrane disruption in vitro translates to mild effects in a randomized placebo-controlled clinical trial.
Meister, Toni Luise; Gottsauner, Josef-Maximilian; Schmidt, Barbara; Heinen, Natalie; Todt, Daniel; Audebert, Franz; Buder, Felix; Lang, Henriette; Gessner, André; Steinmann, Eike; Vielsmeier, Veronika; Pfaender, Stephanie; Cieplik, Fabian.
  • Meister TL; Department for Molecular & Medical Virology, Ruhr University Bochum, Bochum, Germany.
  • Gottsauner JM; Department of Oral and Maxillofacial Surgery, University Hospital Regensburg, Regensburg, Germany.
  • Schmidt B; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany.
  • Heinen N; Department for Molecular & Medical Virology, Ruhr University Bochum, Bochum, Germany.
  • Todt D; Department for Molecular & Medical Virology, Ruhr University Bochum, Bochum, Germany; European Virus Bioinformatics Center (EVBC), Jena, Germany.
  • Audebert F; Praxiszentrum Alte Mälzerei, Regensburg, Germany.
  • Buder F; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany.
  • Lang H; Praxiszentrum Alte Mälzerei, Regensburg, Germany.
  • Gessner A; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany.
  • Steinmann E; Department for Molecular & Medical Virology, Ruhr University Bochum, Bochum, Germany.
  • Vielsmeier V; Department of Otorhinolaryngology, University Hospital Regensburg, Regensburg, Germany. Electronic address: veronika.vielsmeier@ukr.de.
  • Pfaender S; Department for Molecular & Medical Virology, Ruhr University Bochum, Bochum, Germany. Electronic address: stephanie.pfaender@ruhr-uni-bochum.de.
  • Cieplik F; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany. Electronic address: fabian.cieplik@ukr.de.
Virus Res ; 316: 198791, 2022 07 15.
Article in English | MEDLINE | ID: covidwho-1815257
ABSTRACT
The emergence of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) represents an unprecedented threat for the human population, necessitating rapid and effective intervention measures. Given the main infection route by airborne transmission, significant attention has been bestowed upon the use of antiseptic mouthrinses as a way to possibly reduce infectious viral titers. However, clinical evaluations are still sparse. Thus, we evaluated a wide variety of antiseptic agents that can be used as mouthrinses for their antiviral effects in vitro and their respective mode of action. One of the most promising antiseptic agents (benzalkoniumchloride, BAC) was used in a randomized placebo-controlled clinical trial with subsequent analysis of viral loads by RT-qPCR and virus rescue in cell culture. Mechanistic analysis revealed that treatment with BAC and other antiseptic agents efficiently inactivated SARS-CoV-2 in vitro by primarily disrupting the viral envelope, without affecting viral RNA integrity. However, the clinical application only resulted in a mild reduction of viral loads in the oral cavity. These results indicate that gargling with mouthrinses comprising single antiseptic agents may play a minor role towards a potential reduction of transmission rates and thus, these findings are of utmost importance when considering alternative COVID-19 prevention strategies.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Drug Treatment / Anti-Infective Agents, Local Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2022 Document Type: Article Affiliation country: J.virusres.2022.198791

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Drug Treatment / Anti-Infective Agents, Local Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2022 Document Type: Article Affiliation country: J.virusres.2022.198791