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Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants.
Zhang, Jason Z; Yeh, Hsien-Wei; Walls, Alexandra C; Wicky, Basile I M; Sprouse, Kaitlin R; VanBlargan, Laura A; Treger, Rebecca; Quijano-Rubio, Alfredo; Pham, Minh N; Kraft, John C; Haydon, Ian C; Yang, Wei; DeWitt, Michelle; Bowen, John E; Chow, Cameron M; Carter, Lauren; Ravichandran, Rashmi; Wener, Mark H; Stewart, Lance; Veesler, David; Diamond, Michael S; Greninger, Alexander L; Koelle, David M; Baker, David.
  • Zhang JZ; Department of Biochemistry, University of Washington, Seattle, WA, USA. jason.zhang0428@gmail.com.
  • Yeh HW; Institute for Protein Design, University of Washington, Seattle, WA, USA. jason.zhang0428@gmail.com.
  • Walls AC; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Wicky BIM; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Sprouse KR; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • VanBlargan LA; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Treger R; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Quijano-Rubio A; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Pham MN; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Kraft JC; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA.
  • Haydon IC; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Yang W; Department of Bioengineering, University of Washington, Seattle, WA, USA.
  • DeWitt M; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Bowen JE; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Chow CM; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Carter L; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Ravichandran R; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Wener MH; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Stewart L; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Veesler D; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Diamond MS; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Greninger AL; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Koelle DM; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Baker D; Institute for Protein Design, University of Washington, Seattle, WA, USA.
Nat Biotechnol ; 40(9): 1336-1340, 2022 09.
Article in English | MEDLINE | ID: covidwho-1815566
ABSTRACT
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Nat Biotechnol Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: S41587-022-01280-8

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Nat Biotechnol Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: S41587-022-01280-8