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Impact of Heat Inactivation of Blood Samples on Therapeutic Drug Monitoring of 5 Second-Generation Antipsychotics and Their Metabolites.
Ding, Jing; Yang, Liu; Zhang, Yan; Zhang, Suo; Meng, Zhuocheng.
  • Ding J; Pharmacy Laboratory, Xi'an Mental Health Center, Xi'an, P.R. China; and.
  • Yang L; Xi'an Key Laboratory of Pharmacy (Mental Health), Xi'an, P.R. China.
  • Zhang Y; Pharmacy Laboratory, Xi'an Mental Health Center, Xi'an, P.R. China; and.
  • Zhang S; Xi'an Key Laboratory of Pharmacy (Mental Health), Xi'an, P.R. China.
  • Meng Z; Pharmacy Laboratory, Xi'an Mental Health Center, Xi'an, P.R. China; and.
Ther Drug Monit ; 44(5): 701-706, 2022 10 01.
Article in English | MEDLINE | ID: covidwho-1816293
ABSTRACT

BACKGROUND:

The severe acute respiratory syndrome coronavirus 2 outbreak has been classified as a pandemic. Because many coronaviruses are heat sensitive, heat inactivation of patient samples at 56°C before testing reduces the risk of transmission. The aim of this study is to assess the impact of heat inactivation of patient blood samples on plasma concentrations of 5 second-generation antipsychotics and their metabolites.

METHODS:

Blood samples were collected during routine clinical therapeutic drug monitoring examination between April 3, 2021, and April 19, 2021. Samples were divided into 2 groups group A, noninactivated raw sample, and group B, inactivated samples. Inactivation was performed by a 30-minute incubation at 56°C. The levels of the 5 drugs and their metabolites before and after sample heat inactivation were measured using liquid chromatography-tandem mass spectrometry and compared. Furthermore, correlation and Bland-Altman analyses were conducted.

RESULTS:

No statistically significant difference was observed between the levels of the 5 drugs and their metabolites (ie, risperidone, 9-OH-risperidone, aripiprazole, dehydroaripiprazole, olanzapine, quetiapine, norquetiapine, clozapine, and norclozapine) in the noninactivated group A and the inactivated group B ( P > 0.05). Each drug's concentration values in inactivated and noninactivated treatments correlated (Spearman rs > 0.98; P < 0.001). The results of the noninactivated treatment methods and samples alone showed good consistency via Bland-Altman analysis.

CONCLUSIONS:

Blood sample heat inactivation had no significant effect on the therapeutic drug monitoring of 5 second-generation antipsychotics and their metabolites. This inactivated treatment method should be recommended to effectively protect laboratory staff from virus contamination.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Antipsychotic Agents / COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Ther Drug Monit Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antipsychotic Agents / COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Ther Drug Monit Year: 2022 Document Type: Article