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Patients with severe COVID-19 do not have elevated autoantibodies against common diagnostic autoantigens.
Ulndreaj, Antigona; Wang, Mingyue; Misaghian, Salvia; Paone, Louis; Sigal, George B; Stengelin, Martin; Campbell, Christopher; Van Nynatten, Logan R; Soosaipillai, Antoninus; Ghorbani, Atefeh; Mathew, Anu; Fraser, Douglas D; Diamandis, Eleftherios P; Prassas, Ioannis.
  • Ulndreaj A; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.
  • Wang M; Meso Scale Diagnostics, LLC. (MSD), Rockville, MD, USA.
  • Misaghian S; Meso Scale Diagnostics, LLC. (MSD), Rockville, MD, USA.
  • Paone L; Meso Scale Diagnostics, LLC. (MSD), Rockville, MD, USA.
  • Sigal GB; Meso Scale Diagnostics, LLC. (MSD), Rockville, MD, USA.
  • Stengelin M; Meso Scale Diagnostics, LLC. (MSD), Rockville, MD, USA.
  • Campbell C; Meso Scale Diagnostics, LLC. (MSD), Rockville, MD, USA.
  • Van Nynatten LR; Lawson Health Research Institute, London, ON, Canada.
  • Soosaipillai A; Department of Pediatrics, Clinical Neurological Sciences and Physiology and Pharmacology, Western University, London, ON, Canada.
  • Ghorbani A; Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada.
  • Mathew A; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
  • Fraser DD; Meso Scale Diagnostics, LLC. (MSD), Rockville, MD, USA.
  • Diamandis EP; Lawson Health Research Institute, London, ON, Canada.
  • Prassas I; Department of Pediatrics, Clinical Neurological Sciences and Physiology and Pharmacology, Western University, London, ON, Canada.
Clin Chem Lab Med ; 60(7): 1116-1123, 2022 06 27.
Article in English | MEDLINE | ID: covidwho-1817522
ABSTRACT

OBJECTIVES:

Infection by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative pathogen of coronavirus disease 2019 (COVID-19) presents occasionally with an aberrant autoinflammatory response, including the presence of elevated circulating autoantibodies in some individuals. Whether the development of autoantibodies against self-antigens affects COVID-19 outcomes remains unclear. To better understand the prognostic role of autoantibodies in COVID-19, we quantified autoantibodies against 23 markers that are used for diagnosis of autoimmune disease. To this end, we used serum samples from patients with severe [intensive care unit (ICU)] and moderate (ward) COVID-19, across two to six consecutive time points, and compared autoantibody levels to uninfected healthy and ICU controls.

METHODS:

Acute and post-acute serum (from 1 to 26 ICU days) was collected from 18 ICU COVID-19-positive patients at three to six time points; 18 ICU COVID-19-negative patients (sampled on ICU day 1 and 3); 21 ward COVID-19-positive patients (sampled on hospital day 1 and 3); and from 59 healthy uninfected controls deriving from two cohorts. Levels of IgG autoantibodies against 23 autoantigens, commonly used for autoimmune disease diagnosis, were measured in serum samples using MSD® U-PLEX electrochemiluminescence technology (MSD division Meso Scale Discovery®), and results were compared between groups.

RESULTS:

There were no significant elevations of autoantibodies for any of the markers tested in patients with severe COVID-19.

CONCLUSIONS:

Sample collections at longer time points should be considered in future studies, for assessing the possible development of autoantibody responses following infection with SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Autoimmune Diseases / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Long Covid Limits: Humans Language: English Journal: Clin Chem Lab Med Journal subject: Chemistry, Clinical / Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Cclm-2022-0239

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Autoimmune Diseases / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Long Covid Limits: Humans Language: English Journal: Clin Chem Lab Med Journal subject: Chemistry, Clinical / Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Cclm-2022-0239