Application of 2019 Novel Coronavirus (2019-nCoV) specific antibody detection for suspected cases screening
Chinese Journal of Zoonoses
; 37(12):1102-1107, 2021.
Article
in Chinese
| GIM | ID: covidwho-1818312
ABSTRACT
Comparing the accuracy of antibody detection reagents which based on different principles by the diagnosis of suspected cases of new crown, propose an efficient screening plan for suspected cases, and further provide a scientific basis for establishing a clinical screening path for high-risk populations. For 76 suspected cases of COVID-19 that were transferred to designated hospitals for isolation and observation due to the initial IgM antibody test, nasopharyngeal swab nucleic acid tests, serum trace virus neutralizing antibodies and three commercially available antibody test reagents with different principles were tested, combined with lung CT and the examination and clinical symptoms or other information, comprehensively judge the diagnosis or exclusion of the new crown, and compare the three kinds of serological antibody detection reagents with the clinical judgment results to compare the detection rates of the three detection methods. Through comprehensive judgment, 3 suspected cases are confirmed cases, and 73 suspected cases are excluded cases. Compared with the clinical diagnosis/exclusion results, among the three serological antibody detection methods, the chemiluminescence total antibody kit detect positive for all the 9 serums collected from the 3 confirmed cases in different time points, the detection specificity is 97.26% for 73 excluded cases, it's significantly higher than that of the chemiluminescence method IgM/IgG and the colloidal gold method IgM/IgG Separate testing (the specificity is 73.97% on the first day of admission). The chemiluminescence method for the detection of total antibodies to the 2019-nCoV has high sensitivity and high specificity, and can be used for preliminary screening of antibody detection in suspected cases. For the screening of target populations, the 2019-nCoV nucleic acid test should be performed first. If the nucleic acid test is negative, the screening path with the detection of serum total antibodies as the main indicator should be adopted.
screening; diagnosis; diagnostic techniques; detection; antibody testing; human diseases; antibodies; clinical aspects; IgM; lungs; nasopharynx; neutralization; neutralizing antibodies; symptoms; viral diseases; coronavirus disease 2019; Severe acute respiratory syndrome coronavirus 2; man; Hebei; China; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Northern China; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; screening tests; SARS-CoV-2; antibody detection; antibody tests; clinical picture; viral infections; People's Republic of China
Full text:
Available
Collection:
Databases of international organizations
Database:
GIM
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Zoonoses
Year:
2021
Document Type:
Article
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