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Self-Sampled Gargle Water Direct RT-LAMP as a Screening Method for the Detection of SARS-CoV-2 Infections.
Arbaciauskaite, Skaiste; Babakhani, Pouya; Sandetskaya, Natalia; Vitkus, Dalius; Jancoriene, Ligita; Karosiene, Dovile; Karciauskaite, Dovile; Zablockiene, Birute; Kuhlmeier, Dirk.
  • Arbaciauskaite S; Department of Diagnostics, Fraunhofer Institute for Cell Therapy and Immunology IZI, Perlickstraße 1, 04103 Leipzig, Germany.
  • Babakhani P; Institute of Cell Biology and Neurobiology, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
  • Sandetskaya N; Department of Computer Science, University of Bath, Claverton Down, Bath BA2 7AY, UK.
  • Vitkus D; Department of Diagnostics, Fraunhofer Institute for Cell Therapy and Immunology IZI, Perlickstraße 1, 04103 Leipzig, Germany.
  • Jancoriene L; Institute of Biomedical Sciences, Vilnius University Faculty of Medicine, M.K. Ciurlionio 21, LT-03101 Vilnius, Lithuania.
  • Karosiene D; Centre of Laboratory Medicine, Vilnius University Hospital Santaros Klinikos, Santariskiu 14, LT-08406 Vilnius, Lithuania.
  • Karciauskaite D; Clinic of Infectious Diseases and Dermatovenerology, Institute of Clinical Medicine, Vilnius University Faculty of Medicine, M.K. Ciurlionio 21, LT-03101 Vilnius, Lithuania.
  • Zablockiene B; Center of Infectious Diseases, Vilnius University Hospital Santaros Klinikos, Santariskiu 14, LT-08406 Vilnius, Lithuania.
  • Kuhlmeier D; Centre of Laboratory Medicine, Vilnius University Hospital Santaros Klinikos, Santariskiu 14, LT-08406 Vilnius, Lithuania.
Diagnostics (Basel) ; 12(4)2022 Mar 22.
Article in English | MEDLINE | ID: covidwho-1820197
ABSTRACT
We assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-nasopharyngeal swab samples. We also assessed the impact of symptom onset to test time (STT)-i.e., symptom days at sampling, on LAMP. In addition, we appraised the viability of gargle water self-sampling versus oro-nasopharyngeal swab sampling, by comparing paired indirect RT-PCR results. 202 oro-nasopharyngeal swab and paired self-sampled gargle water samples were collected from hospital patients with COVID-19 associated symptoms. LAMP, indirect and direct RT-PCR were performed on all gargle water samples, and indirect RT-PCR was performed on all oro-nasopharyngeal samples. LAMP presented a sensitivity of 80.8% (95% CI 70.8-90.8%) for sample pairs with sub-25 Ct oro-nasopharyngeal indirect RT-PCR results, and 77.6% (66.2-89.1%) sensitivity for sub-30 Ct samples with STT ≤ 7 days. STT, independently of Ct value, correlated negatively with LAMP performance. 80.7% agreement was observed between gargle water and oro-nasopharyngeal indirect RT-PCR results. In conclusion, LAMP presents an acceptable sensitivity for low Ct and low STT samples. Gargle water may be considered as a viable sampling method, and LAMP as a screening method, especially for symptomatic persons with low STT values.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Year: 2022 Document Type: Article Affiliation country: Diagnostics12040775

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Year: 2022 Document Type: Article Affiliation country: Diagnostics12040775