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Effect of Short Time of SARS-CoV-2 Infection in Caco-2 Cells.
Zupin, Luisa; Fontana, Francesco; Clemente, Libera; Ruscio, Maurizio; Ricci, Giuseppe; Crovella, Sergio.
  • Zupin L; Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34137 Trieste, Italy.
  • Fontana F; Division of Laboratory Medicine, University Hospital Giuliano Isontina, 34149 Trieste, Italy.
  • Clemente L; Division of Laboratory Medicine, University Hospital Giuliano Isontina, 34149 Trieste, Italy.
  • Ruscio M; Division of Laboratory Medicine, University Hospital Giuliano Isontina, 34149 Trieste, Italy.
  • Ricci G; Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34137 Trieste, Italy.
  • Crovella S; Department of Medicine, Surgery and Health Sciences, University of Trieste, 34129 Trieste, Italy.
Viruses ; 14(4)2022 03 29.
Article in English | MEDLINE | ID: covidwho-1820405
ABSTRACT
Coronavirus disease 19 (COVID-19) clinical manifestations include the involvement of the gastrointestinal tract, affecting around 10% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected children. In the present work, the consequence of a short time of viral absorption (5, 15, 30 and 60 min) was tested on the Caco-2 intestinal epithelial cell line. Our findings show that Caco-2 cells are highly permissive to SARS-CoV-2 infection, even after 5 min of viral inoculation at a multiplicity of infection of 0.1. No cytopathic effect was evident during the subsequent 7 days of monitoring; nevertheless, the immunofluorescence staining for the viral nucleocapsid confirmed the presence of intracellular SARS-CoV-2. Our findings highlight the very short time during which SARS-CoV-2 is able to infect these cells in vitro.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies / Prognostic study Limits: Child / Humans Language: English Year: 2022 Document Type: Article Affiliation country: V14040704

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies / Prognostic study Limits: Child / Humans Language: English Year: 2022 Document Type: Article Affiliation country: V14040704