Spherical neutralizing aptamer suppresses SARS-CoV-2 Omicron escape.
Nano Today
; 44: 101499, 2022 Jun.
Article
in English
| MEDLINE | ID: covidwho-1821422
ABSTRACT
Recently, the SARS-CoV-2 Omicron has spread very quickly worldwide. Several studies have indicated that the Omicron variant causes a substantial evasion of the humoral immune response and the majority of existing SARS-CoV-2 neutralizing antibodies. Here we address this challenge by applying a spherical cocktail neutralizing aptamer-gold nanoparticle (SNAP) to block the interaction of Omicron receptor binding domain (RBD) and host Angiotensin-Converting Enzyme 2 (ACE2). With the synergetic blocking strategy based on multivalent multisite aptamer binding and steric hindrance by the size-matched gold scaffold, the SNAP conjugate tightly binds to Omicron RBD with a dissociation constant of 13.6 pM, almost completely blocking the infection of Omicron pseudovirus with a half-maximal inhibitory concentration of 35.9 pM. Overall, the SNAP strategy not only fills the gap of the humoral immune evasion caused by clustered mutations on Omicron, but also provides a clue for the development of new broad neutralizing reagents against future variants.
Full text:
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Collection:
International databases
Database:
MEDLINE
Topics:
Variants
Language:
English
Journal:
Nano Today
Year:
2022
Document Type:
Article
Affiliation country:
J.nantod.2022.101499
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