DIGITAL MICROFLUIDIC CHIP BASED ON DIRECT INK WRITING FOR NUCLEIC ACID MULTIPLEX PCR DETECTION
35th IEEE International Conference on Micro Electro Mechanical Systems Conference (IEEE MEMS)
; : 365-368, 2022.
Article
in English
| Web of Science | ID: covidwho-1822038
ABSTRACT
This study presents a facile route to fabricate a novel kind of digital microfluidic (DMF) chip via direct ink writing. The manufacture of this device does not rely on conventional microfabrication processes and cleanrooms, which makes it easy to prepare and low cost. By measuring the change of contact angle (CA) and droplet velocity, we proved that it could perform droplet manipulation like traditional DMF chips. In addition, after optimizing the chip structure, through a peripheral support circuit, polymerase chain reaction (PCR) reagents could be automatically partitioned and mixed on the chip. Furthermore, we realized the multi-target end-point fluorescence detection of SARS-CoV-2 RNA on this chip, showing promising potential for automatic nucleic acid tests.
Full text:
Available
Collection:
Databases of international organizations
Database:
Web of Science
Language:
English
Journal:
35th IEEE International Conference on Micro Electro Mechanical Systems Conference (IEEE MEMS)
Year:
2022
Document Type:
Article
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