Design of a multi-epitope-based peptide vaccine against the S and N proteins of SARS-COV-2 using immunoinformatics approach
Egyptian Journal of Medical Human Genetics
; 23(1), 2022.
Article
in English
| EMBASE | ID: covidwho-1822226
ABSTRACT
Background:
As the new pandemic created by COVID-19 virus created the need of rapid acquisition of a suitable vaccine against SARS-CoV-2 to develop Immunity and to reduce the mortality, the aim of this study was to identify SARS-CoV-2 S protein and N antigenic epitopes by using immunoinformatic methods to design a vaccine against SARS-CoV-2, for which S and N protein-dependent epitopes are predicted. B cell, CTL and HTL were determined based on antigenicity, allergenicity and toxicity that were non-allergenic, non-toxic, and antigenic and were selected for the design of a multi-epitope vaccine structure. Then, in order to increase the safety of Hbd-3 and Hbd-2 as adjuvants, they were connected to the N and C terminals of the vaccine construct, respectively, with a linker. The three-dimensional structure of the structure was predicted and optimized, and its quality was evaluated. The vaccine construct was ligated to MHCI. Finally, after optimizing the codon to increase expression in E. coli K12, the vaccine construct was cloned into pET28a (+) vector.Results:
Epitopes which were used in our survey were based on non-allergenic, non-toxic and antigenic. Therefore, 543-amino-acid-long multi-epitope vaccine formation was invented through linking 9 cytotoxic CTL, 5 HTL and 14 B cell epitopes with appropriate adjuvants and connectors that can control the SARS coronavirus 2 infection and could be more assessed in medical scientific researches.Conclusion:
We believe that the proposed multi-epitope vaccine can effectively evoke an immune response toward SARS-CoV-2.
beta defensin 2; epitope; guanine nucleotide binding protein; peptide vaccine; vitronectin; allergenicity; amino terminal sequence; antigenicity; article; artificial neural network; carboxy terminal sequence; controlled study; coronavirus disease 2019; cytotoxic T lymphocyte; drug design; drug solubility; Escherichia coli; gene frequency; human; human cell; immune response; immunoinformatics; molecular docking; molecular dynamics; protein structure; Severe acute respiratory syndrome coronavirus 2; stereochemistry; vaccine immunogenicity
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Topics:
Vaccines
Language:
English
Journal:
Egyptian Journal of Medical Human Genetics
Year:
2022
Document Type:
Article
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