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Evaluation of conventional and point-of-care real-time RT-PCR tests for the detection of SARS-CoV-2 through a pooled testing strategy.
Yang, Ji-Rong; Kuo, Chuan-Yi; Huang, Hsiang-Yi; Yu, I-Ling; Hsieh, Chih-Tsun; Chen, Bao-Shan; Liu, Ming-Tsan.
  • Yang JR; Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
  • Kuo CY; Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
  • Huang HY; Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
  • Yu IL; Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
  • Hsieh CT; Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
  • Chen BS; Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
  • Liu MT; Center for Diagnostics and Vaccine Development, Centers for Disease Control, Taipei, Taiwan.
J Clin Lab Anal ; 36(6): e24491, 2022 Jun.
Article in English | MEDLINE | ID: covidwho-1826010
ABSTRACT

BACKGROUND:

The rapid identification and isolation of individuals infected with SARS-CoV-2 are fundamental countermeasures for the efficient control of the COVID-19 pandemic, which has affected millions of people around the world. Real-time RT-PCR is one of the most commonly applied reference methods for virus detection, and the use of pooled testing has been proposed as an effective way to increase the throughput of routine diagnostic tests. However, the clinical applicability of different types of real-time RT-PCR tests in a given group size remains inconclusive due to inconsistent regional disease prevalence and test demands.

METHODS:

In this study, the performance of one dual-target conventional and two point-of-care real-time RT-PCR tests in a 5-specimen pooled testing strategy for the detection of SARS-COV-2 was evaluated.

RESULTS:

We demonstrated the proof of concept that all of these real-time RT-PCR tests could feasibly detect SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens that contain viral RNA loads in the range of 3.48 × 105 to 3.42 × 102 copies/ml through pooled testing in a group size of 5 with overall positive percent agreement (pooling vs. individual testing) ranging from 100% to 93.75%. Furthermore, the two POC real-time RT-PCR tests exhibited comparable sensitivity to that of the dual-target conventional one when clinical specimens were tested individually.

CONCLUSION:

Our findings support the feasibility of using real-time RT-PCR tests developed as a variety of platforms in routine laboratory detection of suspected COVID-19 cases through a pooled testing strategy that is beneficial to increasing the daily diagnostic capacity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Humans Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Jcla.24491

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Humans Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Jcla.24491