The role of toothbrush in the transmission of corona- and influenza viruses - results of an in vitro study.
Clin Oral Investig
; 26(9): 5741-5749, 2022 Sep.
Article
in English
| MEDLINE | ID: covidwho-1826515
ABSTRACT
OBJECTIVES:
The aim of this in vitro study was to investigate viruses' stabilities on manual toothbrushes using feline coronavirus (FeCoV) as representative of coronaviruses and an Avian influenza A virus H1N1 for influenza viruses. MATERIAL ANDMETHODS:
Two viruses, FeCoV (Strain Munich; titer 107.5 TCID50/ml) and H1N1 (RE 230/90; titer 106.5 TCID50/ml), were used in this study. Manual toothbrushes were disassembled into bristles, bristle fixation, and back of the toothbrush head, contaminated with the viruses and air-dried for 24 h. In a second experiment, whole toothbrush heads were contaminated, rinsed with water (5 ml for 15 s) and then air-dried.RESULTS:
For FeCoV, immediately after contamination, the following average titers were recovered fixation 106.41, back of head 106.81 and bristles 106.63 TCID50/ml. Following air-drying of 12 (fixation) and 24 h, titers of ≤ 102.5, 103.75, and 102.72 TCID50/ml were found in the respective groups, with a detection limit of 102.5 TCID50/ml. For H1N1, immediately after contamination, the following average titers could be recovered fixation 105.53, back of head 105.97 and bristles 105.75 TCID50/ml. Following air-drying of 8 (fixation) and 24 h, titers were ≤ 102.5, 103.63, and 103.53 TCID50/ml in the respective group, again with 102.5 TCID50/ml being the detection limit. In case of water rinse, no infectious virus could be recovered after 12 h.CONCLUSION:
Viral load of both viruses is reduced by air-drying, especially following water rinsing. Clinical relevance The toothbrush itself plays an insignificant role in the self-transmission of coronavirus and influenza virus.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Influenza A Virus, H1N1 Subtype
Type of study:
Experimental Studies
/
Prognostic study
/
Randomized controlled trials
Language:
English
Journal:
Clin Oral Investig
Journal subject:
Dentistry
Year:
2022
Document Type:
Article
Affiliation country:
S00784-022-04530-w
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