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Comparison of two T cell assays to evaluate T cell responses to SARS-CoV-2 following vaccination in naïve and convalescent healthcare workers.
Phillips, Eloise; Adele, Sandra; Malone, Tom; Deeks, Alexandra; Stafford, Lizzie; Dobson, Susan L; Amini, Ali; Skelly, Donal; Eyre, David; Jeffery, Katie; Conlon, Christopher P; Dold, Christina; Otter, Ashley; Arcangelo, Silvia D'; Turtle, Lance; Klenerman, Paul; Barnes, Eleanor; Dunachie, Susanna J.
  • Phillips E; Peter Medawar Building for Pathogen Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK.
  • Adele S; Peter Medawar Building for Pathogen Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK.
  • Malone T; Peter Medawar Building for Pathogen Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK.
  • Deeks A; Peter Medawar Building for Pathogen Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK.
  • Stafford L; Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, UK.
  • Dobson SL; Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, UK.
  • Amini A; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, UK.
  • Skelly D; Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, UK.
  • Eyre D; Translational Gastroenterology Unit, University of Oxford, Oxford, UK.
  • Jeffery K; Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, UK.
  • Conlon CP; Nuffield Department of Clinical Neuroscience, University of Oxford, UK.
  • Dold C; Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, UK.
  • Otter A; Big Data Institute, University of Oxford, Oxford, UK.
  • Arcangelo S; Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, UK.
  • Turtle L; Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Klenerman P; Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, UK.
  • Barnes E; Oxford Centre For Global Health Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK.
  • Dunachie SJ; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Clin Exp Immunol ; 2022 May 06.
Article in English | MEDLINE | ID: covidwho-1831028
ABSTRACT
T cell responses to SARS-CoV-2 following infection and vaccination are less characterised than antibody responses, due to a more complex experimental pathway.We measured T cell responses in 108 healthcare workers (HCWs) using the commercialised Oxford Immunotec T-SPOT Discovery SARS-CoV-2 assay service (OI T-SPOT) and the PITCH ELISpot protocol established for academic research settings. Both assays detected T cell responses to SARS-CoV-2 spike, membrane and nucleocapsid proteins. Responses were significantly lower when reported by OI T-SPOT than by PITCH ELISpot. Four weeks after two doses of either Pfizer/BioNTech BNT162b or ChAdOx1 nCoV-19 AZD1222 vaccine, the responder rate was 63% for OI T-SPOT Panels1 + 2 (peptides representing SARS-CoV-2 spike protein excluding regions present in seasonal coronaviruses), 69% for OI T-SPOT Panel 14 (peptides representing the entire SARS-CoV-2 spike), and 94% for the PITCH ELISpot assay. The two OI T-SPOT panels correlated strongly with each other showing that either readout quantifies spike-specific T cell responses, although the correlation between the OI T-SPOT panels and the PITCH ELISpot was moderate. The standardisation, relative scalability and longer interval between blood acquisition and processing are advantages of the commercial OI T-SPOT assay. However, the OI T-SPOT assay measures T cell responses at a significantly lower magnitude compared to the PITCH ELISpot assay, detecting T cell responses in a lower proportion of vaccinees. This has implications for the reporting of low-level T cell responses that may be observed in patient populations and for the assessment of T cell durability after vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Cei

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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Cei