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mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients.
Bagheri-Hosseinabadi, Zahra; Rezazadeh Zarandi, Ebrahim; Mirabzadeh, Mohammad; Amiri, Ali; Abbasifard, Mitra.
  • Bagheri-Hosseinabadi Z; Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
  • Rezazadeh Zarandi E; Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
  • Mirabzadeh M; Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
  • Amiri A; Department of Microbiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
  • Abbasifard M; Student Research Committee, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
BMC Infect Dis ; 22(1): 448, 2022 May 10.
Article in English | MEDLINE | ID: covidwho-1833287
ABSTRACT

BACKGROUND:

The etiopathogenesis of coronavirus disease 2019 (COVID-19) stem partially from the abnormal activation of the innate and adaptive immune systems. Here in the current investigation, the mRNA expression levels of toll-like receptors (TLRs) were evaluated in the nasopharyngeal epithelial cells from COVID-19 patients.

METHODS:

Epithelial cells were obtained using nasopharyngeal swab samples from 90 COVID-19 patients and 50 controls. COVID-19 cases were classified into those without symptoms, with symptoms but not hospitalized, and with symptoms and hospitalized. To determine the mRNA expression levels of TLRs, first RNA was extracted and cDNA was synthesized, and finally Real-time PCR was exerted.

RESULTS:

It was seen that the transcript levels of TLR3, TLR7, TLR8, and TLR9 were overexpressed in the COVID-19 patients with clinical symptoms needing hospitalization as well as in those with clinical symptoms without needing for hospitalization compared to controls. Upregulation of TLRs was associated with clinical presentations of the patients.

CONCLUSIONS:

Modulation of TLR3, TLR7, TLR8, TLR9 in the epithelial cells of COVID-19 cases may estimate the disease severity and requirement for hospitalization.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Toll-Like Receptor 3 / COVID-19 Type of study: Experimental Studies / Observational study / Prognostic study Limits: Humans Language: English Journal: BMC Infect Dis Journal subject: Communicable Diseases Year: 2022 Document Type: Article Affiliation country: S12879-022-07437-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Toll-Like Receptor 3 / COVID-19 Type of study: Experimental Studies / Observational study / Prognostic study Limits: Humans Language: English Journal: BMC Infect Dis Journal subject: Communicable Diseases Year: 2022 Document Type: Article Affiliation country: S12879-022-07437-9