Rapid Method to Quantify the Antiviral Potential of Porous and Nonporous Material Using the Enveloped Bacteriophage Phi6.
Environ Sci Technol
; 56(12): 8350-8362, 2022 06 21.
Article
in English
| MEDLINE | ID: covidwho-1838051
ABSTRACT
The pandemic revealed significant gaps in our understanding of the antiviral potential of porous textiles used for personal protective equipment and nonporous touch surfaces. What is the fate of a microbe when it encounters an abiotic surface? How can we change the microenvironment of materials to improve antimicrobial properties? Filling these gaps requires increasing data generation throughput. A method to accomplish this leverages the use of the enveloped bacteriophage Ï6, an adjustable spacing multichannel pipette, and the statistical design opportunities inherent in the ordered array of the 24-well culture plate format, resulting in a semi-automated small drop assay. For 100 mm2 nonporous coupons of Cu and Zn, the reduction in Ï6 infectivity fits first-order kinetics, resulting in half-lives (T50) of 4.2 ± 0.1 and 29.4 ± 1.6 min, respectively. In contrast, exposure to stainless steel has no significant effect on infectivity. For porous textiles, differences associated with composition, color, and surface treatment of samples are detected within 5 min of exposure. Half-lives for differently dyed Zn-containing fabrics from commercially available masks ranged from 2.1 ± 0.05 to 9.4 ± 0.2 min. A path toward full automation and the application of machine learning techniques to guide combinatorial material engineering is presented.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Antiviral Agents
/
Bacteriophages
Language:
English
Journal:
Environ Sci Technol
Year:
2022
Document Type:
Article
Affiliation country:
Acs.est.1c07716
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