Your browser doesn't support javascript.
IgG antibody titers against SARS-CoV-2 nucleocapsid protein correlate with the severity of COVID-19 patients.
Yang, Li; Xu, Qiang; Yang, Bin; Li, Jiayu; Dong, Rong; Da, Jingjing; Ye, Zhixu; Xu, Yongjie; Zhou, Hourong; Zhang, Xiangyan; Liu, Lin; Zha, Yan; Yu, Fuxun.
  • Yang L; Medical College, Guizhou University, Guiyang, People's Republic of China. yl12010731@163.com.
  • Xu Q; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Yang B; Medical College, Guizhou University, Guiyang, People's Republic of China.
  • Li J; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Dong R; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Da J; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Ye Z; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Xu Y; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Zhou H; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Zhang X; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Liu L; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • Zha Y; NHC Key Laboratory of Pulmonary Immunological Diseases (Guizhou Provincial People's Hospital), Guiyang, People's Republic of China.
  • Yu F; Department of Central Laboratory, Guizhou Provincial People's Hospital, No. 83 Zhongshan Dong Road, Guiyang, 550002, Guizhou Province, People's Republic of China.
BMC Microbiol ; 21(1): 351, 2021 12 18.
Article in English | MEDLINE | ID: covidwho-1840945
ABSTRACT

BACKGROUND:

The 2019 novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 virus (SARS-CoV-2) is a current worldwide threat for which the immunological features after infection need to be investigated. The aim of this study was to establish a highly sensitive and quantitative detection method for SARS-CoV-2 IgG antibody and to compare the antibody reaction difference in patients with different disease severity.

RESULTS:

Recombinant SARS-CoV-2 nucleocapsid protein was expressed in Escherichia coli and purified to establish an indirect IgG ELISA detection system. The sensitivity of the ELISA was 100% with a specificity of 96.8% and a 98.3% concordance when compared to a colloidal gold kit, in addition, the sensitivity of the ELISA was 100% with a specificity of 98.9% and a 99.4% concordance when compared to a SARS-CoV-2 spike S1 protein IgG antibody ELISA kit. The increased sensitivity resulted in a higher rate of IgG antibody detection for COVID-19 patients. Moreover, the quantitative detection can be conducted with a much higher serum dilution (1400 vs 110, 1400 vs 1100). The antibody titers of 88 patients with differing COVID-19 severity at their early convalescence ranged from 800 to 102,400, and the geometric mean titer for severe and critical cases, moderate cases, asymptomatic and mild cases was 51,203, 20,912, and 9590 respectively.

CONCLUSION:

The development of a highly sensitive ELISA system for the detection of SARS-CoV-2 IgG antibodies is described herein. This system enabled a quantitative study of rSARS-CoV-2-N IgG antibody titers in COVID-19 patients, the occurrence of higher IgG antibody titers were found to be correlated with more severe cases.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / Coronavirus Nucleocapsid Proteins / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Limits: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged Country/Region as subject: Asia Language: English Journal: BMC Microbiol Journal subject: Microbiology Year: 2021 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / Coronavirus Nucleocapsid Proteins / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Limits: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged Country/Region as subject: Asia Language: English Journal: BMC Microbiol Journal subject: Microbiology Year: 2021 Document Type: Article