Bioinformatics analysis of structure and function of S protein of SARS-CoV-2
Genomics and Applied Biology
; 40(Z1):2346-2355, 2021.
Article
in English, Chinese
| GIM | ID: covidwho-1841703
ABSTRACT
It has been found in previous studies that S protein of coronavirus plays a decisive role in invading host cells. In this paper, nucleotide sequence of SARS-CoV-2 S gene and its encoding amino acid sequence were obtained from NCBI. The bioinformatics analysis of S protein was carried out by DNAMAN, DNAStar, Mega 7.0 and some online analysis and prediction websites. The S protein of SARS-CoV-2 and SARS-CoV has high homology, which suggests that the structure and function of S protein of the two strains are similar. S protein contains signal peptide sequence, and the number of potential phosphorylated amino acids is 136, and the other amino acids have high phosphorylation tendency;there are 17 N-glycosylation sites, and it is a membrane protein, containing S1 and S2 domains;in the prediction results of secondary structure, a helix accounted for 28.59%, beta turn accounted for 3.38%, extended strand accounted for 23.25%, and random coil accounted for 44.78%. The quaternary structure shows that the complete S protein is composed of three monomers. This study can provide a theoretical basis for the infection mechanism and specific treatment of SARS-CoV-2.
amino acid sequences; coronavirus disease 2019; bioinformatics; molecular genetics; nucleotide sequences; phosphorylation; signal peptide; Severe acute respiratory syndrome coronavirus 2; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; SARS-CoV-2; protein sequences; biochemical genetics; DNA sequences; signal sequence
Full text:
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Collection:
Databases of international organizations
Database:
GIM
Language:
English
/
Chinese
Journal:
Genomics and Applied Biology
Year:
2021
Document Type:
Article
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