A Docosahexaenoic Acid Derivative (N-Benzyl Docosahexaenamide) as a Potential Therapeutic Candidate for Treatment of Ovarian Injury in the Mouse Model
Molecules
; 27(9):2754, 2022.
Article
in English
| ProQuest Central | ID: covidwho-1842799
ABSTRACT
Commonly used clinical chemotherapy drugs, such as cyclophosphamide (CTX), may cause injury to the ovaries. Hormone therapies can reduce the ovarian injury risk;however, they do not achieve the desired effect and have obvious side effects. Therefore, it is necessary to find a potential therapeutic candidate for ovarian injury after chemotherapy. N-Benzyl docosahexaenamide (NB-DHA) is a docosahexaenoic acid derivative. It was recently identified as the specific macamide with a high degree of unsaturation in maca (Lepidium meyenii). In this study, the purified NB-DHA was administered intragastrically to the mice with CTX-induced ovarian injury at three dose levels. Blood and tissue samples were collected to assess the regulation of NB-DHA on ovarian function. The results indicated that NB-DHA was effective in improving the disorder of estrous cycle, and the CTX+NB-H group can be recovered to normal levels. NB-DHA also significantly increased the number of primordial follicles, especially in the CTX+NB-M and CTX+NB-H groups. Follicle-stimulating hormone and luteinizing hormone levels in all treatment groups and estradiol levels in the CTX+NB-H group returned to normal. mRNA expression of ovarian development-related genes was positive regulated. The proportion of granulosa cell apoptosis decreased significantly, especially in the CTX+NB-H group. The expression of anti-Müllerian hormone and follicle-stimulating hormone receptor significantly increased in ovarian tissues after NB-DHA treatment. NB-DHA may be a promising agent for treating ovarian injury.
Chemistry--Organic Chemistry; docosahexaenoic acids; ovary; granulosa cells; cyclophosphamide; macamide; Cancer; Side effects; Injury prevention; Severe acute respiratory syndrome coronavirus 2; Follicle-stimulating hormone; Fatty acids; Apoptosis; Ovaries; Chemotherapy; Gene expression; Luteinizing hormone; Reproductive status; Estradiol; Estrus cycle; Follicles; Docosahexaenoic acid; Health risks; Sex hormones
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Collection:
Databases of international organizations
Database:
ProQuest Central
Language:
English
Journal:
Molecules
Year:
2022
Document Type:
Article
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